Spinning Disk confocal microscopes
The platform provides 2 microscopes spinning disk :
1. Spinning Disk inverted
2. Spinning Disk Upright
Spinning Disk Inverted
Main Uses
- fixed and living tissu
- Z-stack, time-lapse
- Acquisition multi-position
- Dye : GFP to Cy3
- Temperature controlled chamber + CO2 controled
Spécification
- Spinning-disk CSUX1-A1 Yokogawa
- Microscope Nikon Ti PFS
- XY motorized stage
- Z stage motorized
- Z piezo 100 µm
- Soft : METAMORPH Premier 7.6
- Caméra Evolve (Type : EMCCD ; 512x512 pixels, pixel size = 16 x 16 µm )
- Objective : 10x Dry, 40x Water, 63x oil and 100x Oil
- Lasers : 405, 491 nm ,561 nm , 640nm
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Spinning Disk Upright
Main Uses
- fixed and living tissu
- z-stack, time-lapse
- Acquisition multi-position
- Dye : DAPI to Cy5
Spécifications
- Spinning-disk CSU10 Yokogawa
- Microscope Leica DM5000B
- XY motorized stage
- Z piezo 200 µm
- Soft : METAMORPH 7,1
- Camera COOLSNAP HQ2
- Objective : 10x dry, 40x, 63x Oil, 100x Oil
- Laser : Melles grilot (400 nm ; 100 mW) ; Cobolt calypso (491 nm ; 50 mW) ; Cobolt jive (561 nm ; 50 mW) ; Cohérent cube (635 nm ; 100 mW)
