Laurent Bourdieu
Cortical Dynamics and Coding Mechanisms
Our group studies neural networks dynamics underlying sensory coding, memory formation and representation of space. We address these questions using advanced optical methods that we improve continuously in terms of sensitivity, temporal resolution, depth penetration or adaptability to freely behaving animals.
We have recently studied the tactile information collected by the rodent whiskers and described how correlated deflections of several whiskers are encoded and mapped in the barrel field of the primary somato-sensory cortex (S1bf). We currently study the formation of expectancies during sensorimotor tasks in S1bf, the formation of memories during sound to reward associations in the primary auditory cortex (A1) and the dynamics of hippocampal networks during spatial exploration.
Recording optically the spiking activity of large cortical networks with cellular resolution is still a technical challenge. Key issues are the depth penetration, the temporal resolution and the sensitivity of the optical recordings. These limitations have led us to develop acousto-optic deflectors (AOD) scanning strategies for two-photon fluorescence microscopy (TPFM). We recently developed and new type of AOD-based microscope 3D AOD scanning with patterned illumination to record from hundreds of cells at a sampling rate larger than 100Hz in awake behaving mice. Finally, our group developed a new generation of fiberscope providing optical sectioning, cellular resolution and fast frame rate imaging for recording and manipulation of neuronal activity in freely behaving rodents.
Selected publications
Uncertainties in signal recovery from heterogeneous and convoluted time series with principal component analysis
Mariia Legenkaia, Laurent Bourdieu, and Rémi Monasson, Phys. Rev. E 111, 044314, https://doi.org/10.1103/PhysRevE.111.044314, arXiv:2412.10175, hal-04826105
Demixing fluorescence time traces transmitted by multimode fibers
C Vaz Rimoli, C Moretti, F Soldevila, E Brémont, C Ventalon, S Gigan, Nature Communications 15, 6286 (2024) arXiv: 2306.00695
Fast wavefront shaping for two-photon brain imaging with large field of view correction
Baptiste Blochet, Walther Akemann, Sylvain Gigan, Laurent Bourdieu.
Proceedings of the National Academy of Sciences 120 (51), e2305593120 (2023) bioRxiv: 2021.09.06.459064
Emergence of time persistence in a data-driven neural network model
S Wolf, G Le Goc, G Debrégeas, S Cocco, R Monasson
2023, Elife 12, e79541 bioRxiv: 2022.02.02.478841
Scattering correcting wavefront shaping for three-photon microscopy
Bernhard Rauer, Hilton B. de Aguiar, Laurent Bourdieu, and Sylvain Gigan, Opt. Letters 47, 6233-6236 (2022) hal-03707080
Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy
Zhuohe Liu, Xiaoyu Lu, Vincent Villette, Yueyang Gou, Kevin L. Colbert, Shujuan Lai , Sihui Guan, Michelle A. Land, Jihwan Lee, Tensae Assefa, Daniel R. Zollinger, Maria M. Korympidou, Anna L. Vlasits, Michelle M. Pang, Sharon Su, Changjia Cai, Emmanouil Froudarakis, Na Zhou, Saumil S. Patel, Cameron L. Smith , François St-Pierre, Cell 185, 3408-3425.e29 (2022)
https://doi.org/10.1016/j.cell.2022.07.013.
Single-shot quantitative aberration and scattering length measurements in mouse brain tissues using an extended-source Shack-Hartmann wavefront sensor
Sophia Imperato, Fabrice Harms, Antoine Hubert, Mathias Mercier, Laurent Bourdieu, Alexandra Fragola. Opt Express. 30:15250-15265 (2022). doi: 10.1364/OE.456651. PMID: 35473251 hal-03608265
Fast optical recording of neuronal activity by three-dimensional custom-access serial holography
Walther Akemann, Sébastien Wolf, Vincent Villette, Benjamin Mathieu, Astou Tangara, Jozsua Fodor, Cathie Ventalon, Jean-François Léger, Stéphane Dieudonné, Laurent Bourdieu, Nat. Methods 19: 100-110 (2022) hal-03606564
Enhanced stability of the focus obtained by wavefront optimization in dynamical scattering media
Baptiste Blochet, Kelly Joaquina, Lisa Blum, Laurent Bourdieu, Sylvain Gigan, Optica 6:1554-1561 (2019) hal-02400519v2
Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice
Vincent Villette, Mariya Chavarha, Ivan K. Dimov, Jonathan Bradley, Lagnajeet Pradhan, Benjamin Mathieu, Stephen W. Evans, Simon Chamberland, Dongqing Shi, Renzhi Yang, Benjamin B. Kim, Annick Ayon,
Abdelali Jalil, Francois St-Pierre, Mark J. Schnitzer, Guoqiang Bi, Katalin Toth, Jun Ding,
Stéphane Dieudonné, and Michael Z. Lin, Cell 179, 1590-1608.e23 (2019)
https://doi.org/10.1016/j.cell.2019.11.004.
Fast confocal fluorescence imaging in freely behaving mice
Clara Dussaux, Vivien Szabo, Yan Chastagnier, Jozsua Fodor, Jean-François Léger, Laurent Bourdieu, Julie Perroy, Cathie Ventalon, Scientific Reports 8, 16262 (2018) hal-02359828v1
Focusing ligth through dynamical samples using fast continuous wavefront optimization
B. Blochet, L. Bourdieu, S. Gigan, Optics Letters, 42:4994-4997 (2017) hal-01797884v1
A radial map of multi-whisker correlation selectivity in the rat barrel cortex
L. Estebanez, J. Bertherat, D. E. Shulz, L. Bourdieu, J.-F. Léger
Nat Commun. 7:13528 (2016) hal-01403564v1 doi: 10.1038/ncomms13528
Fast spatial beam shaping by acousto-optic diffraction for 3D non-linear microscopy.
W. Akemann, J.-F. Léger, C. Ventalon, B. Mathieu, S. Dieudonné, L. Bourdieu
Opt. Exp. 23:28191-28205 (2015) [hal-03619874>
https://hal.archives-ouvertes.fr/hal-03619874]
Characterization of the angular memory effect of scattered light in biological tissues.
S. Schott, J. Bertolotti, J.F. Léger, L. Bourdieu, S. Gigan,
Opt. Exp. 23:13505-13516 (2015) hal-01275498v1
Spatially Selective Holographic Photoactivation and Functional Fluorescence Imaging in Freely Behaving Mice with a Fiberscope.
V. Szabo, C. Ventalon, V. De Sars, J. Bradley, V. Emiliani, Neuron 84:1157–1169 (2014) hal-03619933v1
Late emergence of the vibrissa direction selectivity map in the rat barrel cortex.
Y. Kremer, JF Léger, D Goodman, R Brette, L Bourdieu, J Neurosci. 31:10689-700 (2011) hal-03619888)
