IBENS - ENS

Marie Gendrel

Anne Pizard — 2026-01-09T08:08:16Z

I am a newly recruited ENS associate professor in Biology at IBENS, specialized in Neuroscience

La curiosité intellectuelle a toujours été mon moteur. J'essayais constamment de comprendre comment les choses fonctionnent ; les cours de chimie, physique et biologie sont donc devenus mes sujets de prédilection. Après avoir obtenu mon DEUG de Physique/Chimie à Paris VII, passionnée par les questions fondamentales de Biologie, j'ai décidé d'orienter mes études vers la génétique et la neurobiologie.

Après mon DEUG en Biologie de l'université Paris VI, j'ai été sélectionnée pour rejoindre le Magistère de Génétique en 2002 à l'université Paris VII. J'ai ainsi eu l'opportunité unique d'effecteur plusieurs stages dans des universités et instituts prestigieux. Au cours de la deuxième année de mon Master, j'ai pu suivre le cours Pasteur de Neurosciences à Paris. J'ai alors acquis la certitude que je voulais orienter ma carrière vers les neurosciences en alliant la génétique.

En 2004, pour allier ces deux centres d'intérêts, j'ai rejoint pour mon master puis ma thèse l'équipe de Jean-Louis Bessereau à l'École Normale Supérieure, qui utilise le nématode Caenorhabditis elegans, un ver non parasite pour l'homme, comme modèle génétique afin d'étudier les mécanismes d'agrégation des récepteurs ionotropes à la jonction neuromusculaire. Au cours de ma thèse, j'ai identifié une nouvelle protéine synaptique, LEV-9, qui est nécessaire à la localisation des récepteurs de l'acétylcholine (1). Mes résultats ont mis en évidence un nouveau mécanisme régulant le nombre de récepteurs aux synapses reposant sur des interactions protéine/protéine extracellulaires.

Après avoir étudié les mécanismes cellulaires impliqués dans la formation des synapses, j'ai voulu m'orienter vers la compréhension des différentes étapes du développement du système nerveux et élucider la manière dont un neurone acquiert son identité. En 2010, j'ai ainsi rejoint le laboratoire du Professeur Hobert à l'université de Columbia (New-York, USA) financée par une bourse EMBO puis HFSP. Mon travail, qui m'a permis d'établir qu'un code combinatoire de facteurs de transcription contrôle la différenciation terminale des neurones GABAergiques chez C. elegans, m'a surtout menée à redéfinir le système GABAergique du nématode en identifiant 22 nouvelles cellules contenant du GABA, 15 étant des neurones (4).
Le GABA est le principal neurotransmetteur inhibiteur dans les neurones matures des vertébrés. Que ce soit chez les invertébrés ou les vertébrés, le phénotype GABAergique a été défini de façon traditionnelle par la présence de trois principaux acteurs : (i) GAD, l'enzyme responsable pour la synthèse du GABA à partir du glutamate, (ii) VGAT, le transporteur vésiculaire du GABA (VGAT/unc-47) et (iii) GAT, le transporteur membranaire du GABA qui capture le GABA au niveau de la fente synaptique après sa libération. Cependant, au cours de mon post-doc, j'ai complètement revisité la vision classique que l'on avait du système nerveux GABAergique chez C. elegans et j'ai apporté de nouvelles perspectives quant à ce que définit un neurone GABAergique dans cet organisme modèle. En particulier, j'ai montré que des neurones additionnels contenaient du GABA mais n'exprimaient pas toujours GAD/unc-25, VGAT/unc-47 et GAT/snf-11. En effet, j'ai identifié quatre nouveaux types de neurones qui sont positifs pour le GABA mais qui n'expriment pas toujours l'ensemble des trois acteurs qui définissent le phénotype GABAergique d'un neurone. Deux de ces types montrent de façon flagrante des modes de transport alternatif du GABA puisqu'ils ne possèdent ni VGAT/unc-47 et/ou GAT/snf-11 et ne synthétisent pas de GABA.
Avec deux de mes collègues, nous avons étendu l'étude du système nerveux à celui du mâle qui, en plus de ses 294 neurones en commun avec l'hermaphrodite, possède 93 neurones spécifiques. Nous avons mis en évidence une utilisation différente des neurotransmetteurs selon le sexe de l'animal impliquant donc un changement de fonction des neurones communs selon le sexe (5).

Au cours de ma thèse, j'ai également été recrutée en tant que Moniteur de l'Université Paris VI pour enseigner aux étudiants de Licence la génétique et la biologie cellulaire. A Columbia, j'ai encadré deux étudiants du niveau Master et cinq étudiants du niveau Licence. Trois d'entre eux ont décidé d'effectuer une thèse. Avoir été monitrice ainsi qu'avoir encadré des étudiants ont été des expériences exceptionnelles qui m'ont fait réaliser ma passion et ma capacité d'enseigner.

En 2017, j'ai été recruté comme Maître de Conférences en Neurosciences à l'institut de Biologie de l'Ecole Normale Supérieure (IBENS). J'ai rejoint l'équipe « Transmission inhibitrice » dirigée par Stéphane Dieudonné ce qui me permet d'allier les puissants outils génétiques disponibles chez C. elegans avec le modèle de la souris qui est essentiel pour valider tout mécanisme.
J'ai montré l'existence de mécanismes alternatifs pour le transport du GABA chez C. elegans (4). De plus, chez les neurones dopaminergiques des vertébrés, des observations similaires ont été faites suggérant l'existence de mécanismes alternatifs de transport du GABA. Comprendre ces nouveaux mécanismes apporterait un éclairage nouveau sur la régulation des réseaux neuronaux au travers de l'inhibition. Je propose de tirer avantage de C. elegans, un organisme modèle puissant de par sa génétique, pour identifier et caractériser de nouveaux acteurs présynaptiques de la transmission GABAergique, se concentrant principalement sur des transporteurs supposés ou caractérisés. Nous testerons par la suite les orthologues chez les vertébrés sachant que les composants connus de la machinerie GABAergique sont conservés entre les mammifères et les vers. De nouvelles fonctions pour des transporteurs déjà caractérisés pourraient être découvertes, comme cela a été le cas pour le transporteur vésiculaire du glutamate 1 (VGLUT1) alias BNP1.

PUBLICATIONS

1) Gendrel M, Rapti G, Richmond JE, Bessereau J-L. A secreted complement-control-related protein ensures acetylcholine receptor clustering. Nature. 2009 Oct 15 ;461(7266):992–6.
2) Sancar F, Touroutine D, Gao S, Oh HJ, Gendrel M, Bessereau J-L, Kim H, Zhen M, Richmond JE. The dystrophin-associated protein complex maintains muscle excitability by regulating Ca(2+)-dependent K(+) (BK) channel localization. J Biol Chem. 2011 Sep 23 ;286(38):33501–10.
3) Buonanno M, Garty G, Grad M, Gendrel M, Hobert O, Brenner DJ. Microbeam irradiation of C. elegans nematode in microfluidic channels. Radiat Environ Biophys. 2013 Nov ;52(4):531–7.
4) Gendrel M*, Atlas EG, Hobert O*. A cellular and regulatory map of the GABAergic nervous system of C. elegans. Elife. 2016 Oct 14 ;5:1395. *corresponding author
5) Serrano-Saiz E1*, Pereira L1*, Gendrel M1*, Aghayeva U, Battacharya A, Howell K, Garcia LR, Hobert O*. A Neurotransmitter Atlas of the Caenorhabditis elegans Male Nervous System Reveals Sexually Dimorphic Neurotransmitter Usage. Genetics. 2017 Jul ;206(3):1251–69 1co-first author *corresponding author

Publications

Stéphane Supplisson — 2022-10-20T08:30:00Z

Dynamic role of GlyT1 as glycine sink or source : Pharmacological implications for the gain control of NMDA receptors.
Supplisson S
Neurosci. 2024 Online ahead of print.
doi : 10.1016/j.neuroscience.2024.07.037 PMID : 39059742 PDF

Identification and Organization of a Postural Anti-Gravity Module in the Cerebellar Vermis.
Gouhier A, Vilette V, Mathieu B, Ayon A, Bradley J, Dieudonné S
Neurosci. 2024 Online ahead of print.
doi : 10.1016/j.neuroscience.2024.06.006 PMID : 38897374 PDF

Neurotransmitter content heterogeneity within an interneuron class shapes inhibitory transmission at a central synapse.
Dumontier D, Mailhes-Hamon C, Supplisson S, Dieudonné S.
Front. Cell. Neurosci. 2023 16:1060189.
doi : 10.3389/fncel.2022.1060189 PMID : 36687523 PDF

Flux coupling, not specificity, shapes the transport and phylogeny of SLC6 glycine
transporters.
Le Guellec B, Rousseau F, Bied M, Supplisson S.
PNAS. 2022 119:e2205874119
doi : 10.1073/pnas.2205874119 PMID : 36191186 PDF

Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy.
Liu Z, Lu X, Villette V, Gou Y, Colbert KL, Lai S, Guan S, Land MA, Lee J, Assefa T, Zollinger DR, Korympidou MM, Vlasits AL, Pang MM, Su S, Cai C, Froudarakis E, Zhou N, Patel SS, Smith CL, Ayon A, Bizouard P, Bradley J, Franke K, Clandinin TR, Giovannucci A, Tolias AS, Reimer J, Dieudonné S, St-Pierre F.
Cell. 2022 185:3408-3425
doi : 10.1016/j.cell.2022.07.013 PMID : 35985322

Fast optical recording of neuronal activity by three-dimensional custom-access serial holography.
Akemann W, Wolf S, Villette V, Mathieu B, Tangara A, Fodor J, Ventalon C, Léger JF, Dieudonné S, Bourdieu L.
Nat. Methods. 2022 19:100-110
doi : 10.1038/s41592-021-01329-7 PMID : 34949810

Synaptic Mechanisms Underlying the Network State-Dependent Recruitment of VIP-Expressing Interneurons in the CA1 Hippocampus.
Luo X, Guet-McCreight A , Villette V, Francavilla R, Marino B, Chamberland S, Skinner F K, Topolnik L.
Cereb Cortex. 2020 30:3667-3685
doi : 10.1093/cercor/bhz334 PMID : 32080739

Modular Organization of Cis-regulatory Control Information of Neurotransmitter Pathway Genes in Caenorhabditis elegans.
Serrano-Saiz E, Gulez B, Pereira L, Gendrel M, Kerk SY, Vidal B, Feng W, Wang C, Kratsios P, Rand JB, Hobert O.
Genetics. 2020 215:665-681
doi : 10.1534/genetics.120.303206 PMID : 32444379

Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice.
Villette V, Chavarha M, Dimov IK, Bradley J, Pradhan L, Mathieu B, Evans SW, Chamberland S, Shi D, Yang R, Kim BB, Ayon A, Jalil A, St-Pierre F, Schnitzer MJ, Bi G, Toth K, Ding J, Dieudonné S, Lin MZ.
Cell. 2019 179:1590-1608
doi : 10.1016/j.cell.2019.11.004 PMID : 31835034 PDF

Control of aversion by glycine-gated GluN1/GluN3A NMDA receptors in the adult medial habenula.
Otsu Y, Darcq E, Pietrajtis K, Mátyás F, Schwartz E, Bessaih T, Abi Gerges S, Rousseau CV, Grand T, Dieudonné S, Paoletti P, Acsády L, Agulhon C, Kieffer BL, Diana MA.
Science. 2019 366:250-254
doi : 10.1126/science.aax1522 PMID : 31601771 PDF

Heterogeneous Signaling at GABA and Glycine Co-releasing Terminals.
Aubrey KR, Supplisson S.
Front Synaptic Neurosci. 2018 10:40.
doi : 10.3389/fnsyn.2018.00040 PMID : 30524262 PDF

Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties.
Hernandez O, Pietrajtis K, Mathieu B, Dieudonné S.
Sci Rep. 2018 8(1):13768.
doi : 10.1038/s41598-018-32017-w PMID : 30213968 PDF

Voltage- and Branch-Specific Climbing Fiber Responses in Purkinje Cells.
Zang Y, Dieudonné S, De Schutter E.
Cell Rep. 2018 24(6) :1536-1549.
doi :10.1016/j.celrep.2018.07.011. PMID : 30089264 PDF

Whole-GUV patch-clamping.
Garten M, Mosgaard L, Bornschlögl T, Dieudonné S, Bassereau P, Toombes G.
PNAS. 2017 114(2):328-333.
doi : 10.1073/pnas.1609142114 PMID : 28003462 PDF

Mechanisms and functional roles of glutamatergic synapse diversity in a cerebellar circuit.
Zampini V, Liu J, Diana M, Maldonado P, Brunel N, Dieudonné S,
Elife. 2016 5:e15872
doi : 10.7554/eLife.15872 PMID : 27642013 PDF

Burst-dependent bidirectional plasticity in the cerebellum is driven by presynaptic NMDA receptors.
Bouvier G, Higgins D, Spolidoro M, Carrel D, Mathieu B, Léna C, Dieudonné S, Barbour B, Brunel N, Casado M.
Cell Report. 2016 15(1):104-116
doi : 10.1016/j.celrep.2016.03.004 PMID : 27052175 PDF

Fast spatial beam shaping by acousto-optic diffraction for 3D non-linear microscopy.
Akemann W, Léger J-F, Ventalon C, Mathieu B, Dieudonné S, Bourdieu L.
Opt Express 23(22):28191–28205.
doi : 10.1364/OE.23.028191 PMID : 26561090 PDF

A novel inhibitory nucleo-cortical circuit controls cerebellar Golgi cell activity.
Ankri L, Husson Z, Pietrajtis K, Proville R, Léna C, Yarom Y, Dieudonné S, Yoe Uusisaari M.
Elife. 2015 4:e06262
doi : 10.7554/eLife.06262 PMID : 25965178 PDF

CaRuby-Nano : a novel high affinity calcium probe for dual color imaging.
Collot M, Wilms CD, Bentkhayet A, Marcaggi P, Couchman K, Charpak S, Dieudonné S, Häusser M, Feltz A, Mallet JM.
Elife. 2015 4:e05808
doi : 10.7554/eLife.05808 PMID : 25824291 PDF

A subcortical inhibitory signal for behavioral arrest in the thalamus.
Giber K, Diana MA, M Plattner V, Dugué GP, Bokor H, Rousseau CV, Maglóczky Z, Havas L, Hangya B, Wildner H, Zeilhofer HU, Dieudonné S, Acsády L
Nat Neurosci. 2015 18(4):562-568.
doi : 10.1038/nn.3951 PMID : 25706472 PDF

Activity-dependent gating of calcium spikes by A-type K⁺ channels controls climbing fiber signaling in Purkinje cell dendrites.
Otsu Y, Marcaggi P, Feltz A, Isope P, Kollo M, Nusser Z, Mathieu B, Kano M, Tsujita M, Sakimura K, Dieudonné S.
Neuron. 2014 84(1):137-151.
doi : 10.1016/j.neuron.2014.08.035 PMID : 25220810 PDF

Differential GABAergic and glycinergic inputs of inhibitory interneurons and Purkinje cells to principal cells of the cerebellar nuclei.
Husson Z, Rousseau CV, Broll I, Zeilhofer HU, Dieudonné S.
J Neurosci. 2014 34(28):9418-9431
doi : 10.1523/JNEUROSCI.0401-14.2014 PMID : 25009273 PDF

Cytosolic transmitter concentration regulates vesicle cycling at hippocampal GABAergic terminals.
Wang L, Tu P, Bonet L, Aubrey K, Supplisson S.
Neuron 2013 80(1):143-158
doi : 10.1016/j.neuron.2013.07.021 PMID : 24094108 PDF

Clusters of cerebellar Purkinje cells control their afferent climbing fiber discharge.
Chaumont J, Guyon N, Valera AM, Dugué GP, Popa D, Marcaggi P, Gautheron V, Reibel-Foisset S, Dieudonné S, Stephan A, Barrot M, Cassel JC, Dupont JL, Doussau F, Poulain B, Selimi F, Léna C, Isope P.
PNAS 2013 1140(40):16223-16228
doi : 10.1073/pnas.1302310110 PMID : 24046366 PDF

Granule cell ascending axon excitatory synapses onto Golgi cells implement a potent feedback circuit in the cerebellar granular layer.
Cesana E, Pietrajtis K, Bidoret C, Isope P, D'Angelo E, Dieudonné S, Forti L.
J Neurosci. 2013 33(30):12430-12446
doi : 10.1523/JNEUROSCI.4897-11.2013 PMID : 23884948 PDF

Mutations in the GlyT2 gene (SLC6A5) are a second major cause of startle disease
Carta E, Chung S-K, James VM, Robinson A, Gill JL, Remy N, Vanbellinghen J-F, Drew CJG, Cagdas S, Cameron D, Cowan FM, Del Toro M, Graham GE, Manzur AY, Masri A, Rivera S, Scalais E, Shiang R, Sinclair K, Stuart CA, Tijssen MAJ, Wise G, Zuberi SM, Harvey K, Pearce BR, Topf M, Thomas RH, Supplisson S, Rees MI, Harvey RJ.
J Biol Chem 2012 287(34):28975-28985
doi : 10.1074/jbc.M112.372094 PMID : 22700964 PDF

NMDA receptors with incomplete Mg²⁺ block enable low-frequency transmission through the cerebellar cortex.
Schwartz E, Rothman J, Dugué G, Diana M, Rousseau C, Silver A, Dieudonné S.
J Neurosci 2012 32(20):6878-6893
doi : 10.1523/JNEUROSCI.5736-11.2012 PMID : 22593057 PDF

Mixed inhibitory synaptic balance correlates with glutamatergic synaptic phenotype in cerebellar unipolar brush cells.
Rousseau C, Dugué G, Dumoulin A, Mugnaini E, Dieudonné S, Diana M.
J Neurosci 2012 32(13):4632-4644.
doi : 10.1523/JNEUROSCI.5122-11.2012 PMID : 22457509 PDF

Mechanism of proton/substrate coupling in the heptahelical lysosomal transporter cystinosin
Ruivo R, Bellenchi GC, Chen X, Zifarelli G, Sagne C, Debacker C, Pusch M, Supplisson S, Gasnier B.
PNAS 2012 109(5):E210-E217
doi : 10.1073/pnas.1115581109 PMID : 22232659 PDF

Functional coupling between mGluR1 and Cav_3.1 T-type calcium channels contributes to parallel fiber-induced fast calcium signaling within Purkinje cell dendritic spines.
Hildebrand ME, Isope P, Miyazaki T, Nakaya T, Garcia E, Feltz A, Schneider T, Hescheler J, Kano M, Sakimura K, Watanabe M, Dieudonné S, Snutch TP.
J Neurosci. 2009 29(31):9668-9682
doi : 10.1523/JNEUROSCI.0362-09.2009 PMID : 19657020 PDF

Electrical coupling mediates tunable low-frequency oscillations and resonance in the cerebellar Golgi cell network.
Dugué GP, Brunel N, Hakim V, Schwartz E, Chat M, Lévesque M, Courtemanche R, Léna C, Dieudonné S.
Neuron. 2009 61(1):126-39
doi : 10.1016/j.neuron.2008.11.028 PMID : 19146818 PDF

A critical role for glycine transporters in hyperexcitability disorders.
Harvey RJ, Carta E, Pearce BR, Chung SK, Supplisson S, Rees MI, Harvey K.
Front Mol Neurosci. 2008 1:1
doi : 10.3389/neuro.02.001.2008 PMID : 18946534

The glycine transporter GlyT2 controls the dynamics of synaptic vesicle refilling in inhibitory spinal cord neurons.
Rousseau F, Aubrey KR, Supplisson S.
J Neurosci. 2008 28(39):9755-9768
doi : 10.1523/JNEUROSCI.0509-08.2008 PMID : 18815261 PDF

Optical monitoring of neuronal activity at high frame rate with a digital random-access multiphoton (RAMP) microscope.
Otsu Y, Bormuth V, Wong J, Mathieu B, Dugué GP, Feltz A, Dieudonné S.
J Neurosci Methods. 2008 173(2):259-270.
doi : 10.1016/j.jneumeth.2008.06.015
PMID : 18634822

A spatio-temporally compensated acousto-optic scanner for two-photon microscopy providing large field of view.
Kremer Y, Léger JF, Lapole R, Honnorat N, Candela Y, Dieudonné S, Bourdieu L.
Opt Express. 2008 16(14):10066-10076.
doi :10.1364/OE.16.010066 PMID : 18607414 PDF

The transporters GlyT2 and VIAAT cooperate to determine the vesicular glycinergic phenotype.
Aubrey KR, Rossi FM, Ruivo R, Alboni S, Bellenchi GC, Le Goff A, Gasnier B, Supplisson S.
J Neurosci. 2007 27(23):6273-81.
doi : 10.1523/JNEUROSCI.1024-07.2007 PMID : 17554001 PDF

T-type and L-type Ca²⁺ conductances define and encode the bimodal firing pattern of vestibulocerebellar unipolar brush cells.
Diana MA, Otsu Y, Maton G, Collin T, Chat M, Dieudonné S.
J Neurosci. 2007 27(14):3823-38.
doi : 10.1523/JNEUROSCI.4719-06.2007 PMID : 17409247 PDF

Mutations in the gene encoding GlyT2 (SLC6A5) define a presynaptic component of human startle disease.
Rees M, Harvey K, Pearce B, Chung S-K, Duguid I, Thomas P, Beatty S, Graham G, Armstrong L, Shiang R, Abbott K, Zuberi S, Stephenson J, Owen M, Tijssen M, Maagdenberg A van den, Smart T, Supplisson S, Harvey R
Nat Genet. 2006 38(7):801-6
doi :10.1038/ng1814 PMID:16751771 PDF

Alumni

Stéphane Supplisson — 2022-09-20T09:30:00Z

Scientific staff

Marco Diana (CR CNRS)

Mechanisms and functional roles of glutamatergic synapse diversity in a cerebellar circuit. Zampini V, Liu J, Diana M, Maldonado P, Brunel N, Dieudonné S. eLife (2016) 5.e15872.
A subcortical inhibitory signal for behavioral arrest in the thalamus. Giber K, Diana M, Plattner V, Dugué G, Bokor H, Rousseau C, Maglóczky Z, Havas L, Hangya B, Wildner H, Zeilhofer H, Dieudonné S, Acsády L. Nature Neuroscience (2015) 18:562–568.
NMDA Receptors with Incomplete Mg2+ Block Enable Low-Frequency Transmission through the Cerebellar Cortex. Schwartz E, Rothman J, Dugué G, Diana M, Rousseau C, Silver A, Dieudonné S. J Neurosci (2012) 32:6878-6893
Mixed Inhibitory Synaptic Balance Correlates with Glutamatergic Synaptic Phenotype in Cerebellar Unipolar Brush Cells. Rousseau CV, Dugué G, Dumoulin A, Mugnaini E, Dieudonné S, Diana M. The Journal of Neuroscience (2012) 32:4632–4644.
T-Type and L-Type Ca2+ Conductances Define and Encode the Bimodal Firing Pattern of Vestibulocerebellar Unipolar Brush Cells. Diana M, Otsu Y, Maton G, Collin T, Chat M, Dieudonné S. J Neurosci (2007) 27, 3823–3838

Païkan Marcaggi (CR CNRS)

Activity-dependent gating of calcium spikes by A-type K+ channels controls climbing fiber signaling in Purkinje cell dendrites.Otsu Y, Marcaggi P, Feltz A, Isope P, Kollo M, Nusser Z, Mathieu B, Kano M, Tsujita M, Sakimura K, Dieudonné S. Neuron (2014) 84:137-151
Clusters of cerebellar Purkinje cells control their afferent climbing fiber discharge. Chaumont J, Guyon N, Valera AM, Dugué GP, Popa D, Marcaggi P, Gautheron V, Reibel-Foisset S, Dieudonné S, Stephan A, Barrot M, Cassel JC, Dupont JL, Doussau F, Poulain B, Selimi F, Léna C, Isope P. PNAS (2013) 110:16223-16228

Post-doc

Yo Otsu

Activity-dependent gating of calcium spikes by A-type K+ channels controls climbing fiber signaling in Purkinje cell dendrites. Otsu Y, Marcaggi P, Feltz A, Isope P, Kollo M, Nusser Z, Mathieu B, Kano M, Tsujita M, Sakimura K, Dieudonné S. Neuron (2014) 84:137-151
Optical monitoring of neuronal activity at high frame rate with a digital random-access multiphoton (RAMP) microscope. Otsu Y, Bormuth V, Wong J, Mathieu B, Dugué G, Feltz A, Dieudonné S. J Neurosci Meth (2008) 173, 259–270.
T-Type and L-Type Ca2+ Conductances Define and Encode the Bimodal Firing Pattern of Vestibulocerebellar Unipolar Brush Cells. Diana, M., Otsu Y, Maton, G., Collin, T., Chat, M., Dieudonné, S.The Journal of Neuroscience Diana, M., Otsu, Y., Maton, G., Collin, T., Chat, M., Dieudonné, S. (2007) 27, 3823–3838

Valeria Zampini

Mechanisms and functional roles of glutamatergic synapse diversity in a cerebellar circuit. Zampini V, Liu J, Diana M, Maldonado P, Brunel N, Dieudonné S. eLife (2016) 5.e15872

Eric Schwartz (MC UPMC)

NMDA receptors with incomplete Mg²⁺ block enable low-frequency transmission through the cerebellar cortex. Schwartz E, Rothman J, Dugué G, Diana M, Rousseau C, Silver A, Dieudonné S. J Neurosci (2012) 32:6878-6893
Electrical coupling mediates tunable low-frequency oscillations and resonance in the cerebellar Golgi cell network. Dugué GP, Brunel N, Hakim V, Schwartz E, Chat M, Lévesque M, Courtemanche R, Léna C, Dieudonné S. Neuron (2009) 61:126-139

Peng Tu

Cytosolic Transmitter Concentration Regulates Vesicle Cycling at Hippocampal GABAergic Terminals. Wang* L, Tu P*, Bonet L, Aubrey KR, Supplisson S. Neuron (2013) 80:143-158

Karin Aubrey

Heterogeneous Signaling at GABA and Glycine Co-releasing Terminals. Aubrey KR, Supplisson S. Front Synaptic Neurosci (2018) 6:40
Cytosolic Transmitter Concentration Regulates Vesicle Cycling at Hippocampal GABAergic Terminals. Wang* L, Tu* P, Bonet L, Aubrey KR , Supplisson S. Neuron (2013) 80:143-158
The glycine transporter GlyT2 controls the dynamics of synaptic vesicle refilling in inhibitory spinal cord neurons. Rousseau F, Aubrey KR, Supplisson S. J Neurosci (2008) 28:9755-9768
The transporters GlyT2 and VIAAT cooperate to determine the vesicular glycinergic phenotype. Aubrey KR*, Rossi FM*, Ruivo R, Alboni S, Bellenchi GC, Le Goff A, Gasnier B, Supplisson S. J Neurosci (2007) 27:6273-6281

Francesco Rossi

The transporters GlyT2 and VIAAT cooperate to determine the vesicular glycinergic phenotype. Aubrey KR*, Rossi FM*, Ruivo R, Alboni S, Bellenchi GC, Le Goff A, Gasnier B, Supplisson S. J Neurosci (2007) 27:6273-6281

Ph.D. Students

Dimitri Dumontier

Neurotransmitter content heterogeneity within an interneuron class shapes inhibitory transmission at a central synapse. Dumontier D, Mailhes-Hamon C, Supplisson S, Dieudonné S.
Front. Cell. Neurosci. 2023 16:1060189

Laurine Bonet

Cytosolic Transmitter Concentration Regulates Vesicle Cycling at Hippocampal GABAergic Terminals. Wang* L, Tu* P, Bonet L, Aubrey KR, Supplisson S. Neuron (2013) 80:143-158

Zoé Husson

A novel inhibitory nucleo-cortical circuit controls cerebellar Golgi cell activity.
Ankri L, Husson Z, Pietrajtis K, Proville R, Léna C, Yarom Y, Dieudonné S, Uusisaari M. Elife (2015) 4.e06262.
Differential GABAergic and Glycinergic Inputs of Inhibitory Interneurons and Purkinje Cells to Principal Cells of the Cerebellar Nuclei. Husson Z, Rousseau C, Broll I, Zeilhofer H, Dieudonné S. J Neurosci (2014) 34:9418–9423

Katarzyna Pietrajtis

A novel inhibitory nucleo-cortical circuit controls cerebellar Golgi cell activity.
Ankri L, Husson Z, Pietrajtis K, Proville R, Léna C, Yarom Y, Dieudonné S, Uusisaari M. Elife (2015) 4.e06262
Granule cell ascending axon excitatory synapses onto Golgi cells implement a potent feedback circuit in the cerebellar granular layer. Cesana E, Pietrajtis K, Bidoret C, Isope P, D'Angelo E, Dieudonné S, Forti L. J Neurosci (2013) 33:12430-12446

Charly Rousseau

Differential GABAergic and Glycinergic Inputs of Inhibitory Interneurons and Purkinje Cells to Principal Cells of the Cerebellar Nuclei. Husson Z, Rousseau C, Broll I, Zeilhofer H, Dieudonné S. J Neurosci (2014) 34:9418–9423
Mixed inhibitory synaptic balance correlates with glutamatergic synaptic phenotype in cerebellar unipolar brush cells. Rousseau CV, Dugué GP, Dumoulin A, Mugnaini E, Dieudonné S, Diana MA. J Neurosci (2012) 32:4632-4644
NMDA receptors with incomplete Mg²⁺ block enable low-frequency transmission through the cerebellar cortex. Schwartz EJ, Rothman JS, Dugué GP, Diana M, Rousseau C, Silver RA, Dieudonné S. J Neurosci (2012) 32:6878-6893

Lu Wang

Cytosolic Transmitter Concentration Regulates Vesicle Cycling at Hippocampal GABAergic Terminals. Wang L*, Tu P*, Bonet L, Aubrey KR, Supplisson S. Neuron 2013 80:143-158

Guillaume Dugué (CR CNRS)

NMDA receptors with incomplete Mg²⁺ block enable low-frequency transmission through the cerebellar cortex. Schwartz EJ, Rothman JS, Dugué GP, Diana M, Rousseau C, Silver RA, Dieudonné S. J Neurosci (2012) 32:6878-6893
Mixed inhibitory synaptic balance correlates with glutamatergic synaptic phenotype in cerebellar unipolar brush cells. Rousseau CV, Dugué GP, Dumoulin A, Mugnaini E, Dieudonné S, Diana MA. J Neurosci (2012) 32:4632-4644
Electrical coupling mediates tunable low-frequency oscillations and resonance in the cerebellar Golgi cell network. Dugué GP, Brunel N, Hakim V, Schwartz E, Chat M, Lévesque M, Courtemanche R, Léna C, Dieudonné S. Neuron (2009) 61:126-139
Target-dependent use of co-released inhibitory transmitters at central synapses. Dugué GP, Dumoulin A, Triller A, Dieudonné S. J Neurosci (2005) 25:6490-6498
Optical monitoring of neuronal activity at high frame rate with a digital random-access multiphoton (RAMP) microscope. Otsu Y, Bormuth V, Wong J, Mathieu B, Dugué GP, Feltz A, Dieudonné S. J Neurosci Methods (2008) 173:259-270

France Rousseau

Flux coupling, not specificity, shapes the transport and phylogeny of SLC6 glycine transporters. Le Guellec B, Rousseau F, Bied M, Supplisson S. PNAS 2022 119:e2205874119.
The glycine transporter GlyT2 controls the dynamics of synaptic vesicle refilling in inhibitory spinal cord neurons. Rousseau F, Aubrey KR, Supplisson S. J Neurosci (2008) 28:9755-9768
Striatal progenitors derived from human ES cells mature into DARPP32 neurons in vitro and in quinolinic acid-lesioned rats. Aubry L, Bugi A, Lefort N, Rousseau F, Peschanski M, Perrier AL. PNAS (2008) 105:16707-16712

Michel Roux (CR CNRS)

Why glycine transporters have different stoichiometries. Supplisson S, Roux MJ. FEBS Lett. (2002) 529:93-101
The glial and the neuronal glycine transporters differ in their reactivity to sulfhydryl reagents. Roux MJ, Martinez-Maza R, Le Goff A, Lopez-Corcuera B, Aragon C, Supplisson S. J Biol Chem (2001) 276:17699-17705
Neuronal and glial glycine transporters have different stoichiometries. Roux MJ, Supplisson S. Neuron (2000) 25:373-383
Differential properties of two stably expressed brain-specific glycine transporters. López-Corcuera B, Martínez-Maza R, Núñez E, Roux M, Supplisson S, Aragón C. J Neurochem (1998) 71:2211-2219

Graduate Students

Tristan Charitat M2
Yasmine Hoyeau M2
Albert Buchard M2
Bastien Le Guellec M1 PNAS 2022 119:e2205874119.
Mathieu Tournebize M1
Silvana Valtcheva M1
Julia Erb M1

Regulation of molecular interactions between synaptic components

Sarah Reemers — 2018-01-25T13:36:41Z

Head of Project : Christian Specht
Christian Specht CR Inserm

Single molecule localisation microscopy (SMLM) gives access to spatio-temporal distribution patterns that reflect the molecular interactions in which the proteins engage : we can count the number and density of molecules in specific compartments, visualise their localisation with high spatial precision, and track their movements at high temporal resolution in living cells. The comparison with biochemical approaches shows that the mobility of cellular components is inversely related to the binding constants of purified proteins, demonstrating that SMLM data can yield information about molecular interactions in living cells.

Changes in the number or the binding properties of synaptic proteins necessarily alter the organisation of the synaptic structure, which in turn affects the efficacy of synaptic transmission. Using SMLM strategies our project explores the interactions between membrane receptors, scaffold and adhesion proteins as well as signalling and cytoskeletal elements at synapses. We investigate how the binding properties of different pre- and postsynaptic components are regulated through network activity and signalling processes in cultured neurons and organotypic slices.

Participants : C. Specht, X. Yang, A. Jan, F. Niwa, and N. Frézel

Grünewald N, Jan A, Salvatico C, Kress V, Renner R, Triller A, Specht CG, Schwarz G (2018). Sequences flanking the gephyrin-binding site of GlyR-beta tune receptor stabilization at synapses. eNeuro.

Patrizio A, Renner M, Pizzarelli R, Triller A, Specht CG (2017). Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers. Sci Rep 7:10899.

Salvatico C, Specht CG and Triller A (2015). Synaptic receptor dynamics : from theoretical concepts to deep quantification and chemistry in cellulo. Neuropharmacol 88:2-9.

Specht CG, Izeddin I, Rodriguez PC, El Beheiry M, Rostaing P, Darzacq X, Dahan M and Triller A (2013). Quantitative nanoscopy of inhibitory synapses : counting gephyrin molecules and receptor binding sites. Neuron 79:308-321.

Gaspard Gerschenfeld

Gaspard Gerschenfeld (équipe Charnay) — 2017-11-27T10:44:44Z

Training

2013-2015 Neurology resident
2009-2010 Master Degree in stem cell biology (UPMC)
2007-2013 Medical student (UPMC)

Research topic

Use of skin precursor cells to regenerate the central nervous system

Fanny COULPIER

Gaspard Gerschenfeld (équipe Charnay) — 2015-02-18T09:29:07Z

Fanny COULPIER
IE
01 44 32 39 76
E-Mail

Training

2002 : BTS in biotechnology

2004 : Assistant Engineer at the Ecole Normale Supérieur, INSERM U784, in Patrick Charnay's lab

2004-2007 : « Diploma of EPHE » , at the Ecole Normale Supérieur, INSERM U784 , in Patrick Charnay's lab.

2007-2010 : Ph.D in Development Biology at the Ecole Normale Supérieur, Paris, in Patrick Charnay's lab. Thesis : Functionnal analysis of boundary cap cells ans Schwann cells during the peripheral nervous system development.

2010- : Engineer, at the Ecole Normale Supérieur, Paris, U1024 in Patrick Charnay's lab

Research topics

Molecular and cellular characterisation of boundary cap-derived stem cells in peripheral nervous sytem.

Recent publications

Cthrc1 is a negative regulator of myelination in Schwann cells.
Apra C, Richard L, Coulpier F, Blugeon C, Gilardi-Hebenstreit P, Vallat JM, Lindner V, Charnay P, Decker L.Glia. 2012 Mar ;60(3):393-403.

Boundary cap cells are peripheral nervous system stem cells that can be redirected into central nervous system lineages.
Zujovic V, Thibaud J, Bachelin C, Vidal M, Deboux C, Coulpier F, Stadler N, Charnay P, Topilko P, Baron-Van Evercooren A.
Proc Natl Acad Sci U S A. 2011 Jun 28 ;108(26):10714-9. doi : 10.1073/pnas.1018687108. Epub 2011 Jun 13.

Krox20 inactivation in the PNS leads to CNS/PNS boundary transgression by central glia.
Charnay P, Coulpier F, Decker L, Funalot B, Vallat JM, Garcia-Bragado F, Topilko P.
Bull Acad Natl Med. 2010 Apr-May ;194(4-5):743-4. French.

Secreted amyloid precursor protein β and secreted amyloid precursor protein α induce axon outgrowth in vitro through Egr1 signaling pathway.
Chasseigneaux S, Dinc L, Rose C, Chabret C, Coulpier F, Topilko P, Mauger G, Allinquant B.PLoS One. 2011 Jan 27 ;6(1):e16301. doi : 10.1371/journal.pone.0016301.

Krox20 inactivation in the PNS leads to CNS/PNS boundary transgression by central glia.
Coulpier F, Decker L, Funalot B, Vallat JM, Garcia-Bragado F, Charnay P, Topilko P.
Rev Neurol (Paris). 2011 Jan ;167(1):51-6. doi : 10.1016/j.neurol.2010.07.043. Epub 2010 Dec 28.

CNS/PNS boundary transgression by central glia in the absence of Schwann cells or Krox20/Egr2 function.
Coulpier F, Decker L, Funalot B, Vallat JM, Garcia-Bragado F, Charnay P, Topilko P.
J Neurosci. 2010 Apr 28 ;30(17):5958-67. doi : 10.1523/JNEUROSCI.0017-10.2010.

Boundary cap cells are highly competitive for CNS remyelination : fast migration and efficient differentiation in PNS and CNS myelin-forming cells.
Zujovic V, Thibaud J, Bachelin C, Vidal M, Coulpier F, Charnay P, Topilko P, Baron-Van Evercooren A.
Stem Cells. 2010 Mar 31 ;28(3):470-9. doi : 10.1002/stem.290.

Novel features of boundary cap cells revealed by the analysis of newly identified molecular markers.
Coulpier F, Le Crom S, Maro GS, Manent J, Giovannini M, Maciorowski Z, Fischer A, Gessler M, Charnay P, Topilko P.
Glia. 2009 Oct ;57(13):1450-7. doi : 10.1002/glia.20862.

Inhibitory synapses regulation by glia and inflammation

Andrea Dumoulin (équipe Triller) — 2015-02-11T14:00:47Z

Head of project : Alain Bessis
Alain Bessis DR CNRS

Microglia are non-neuronal cells of the nervous tissue. They display strong phagocytic activity, and the microglial functions that have been best described are related to their ability eliminate dead cells, cellular debris etc. However, it is more and more accepted that microglia are more than “housekeeping cells” but rather are full-time partners of neuronal function.

Thus, microglia are extremely ramified and dynamic cells that permanently extend and retract their processes to scan the brain and rapidly react to the modification of the environment. We and others have shown that microglia are able to induce the physiological death of developing neurons and that they participate to the growth of axons during development.

We showed that microglia are genuine partners of excitatory neurotransmission and we are now investigating how microglia can tune the synaptic function by controlling the behaviour of neurotransmitter receptors. To this aim, we are using up-to-date imaging approaches to monitor receptors dynamic and microglia-synapses interactions.

Participants : A. Bessis, I. Vaccari, Y. Cantaut-Belarif, S. Colasse

Cantaut-Belarif Y, M. Antri, R. Pizzarelli, S. Colasse, I. Vaccari, S. Soares, M. Renner, R. Dallel, A. Triller, and A. Bessis (2017) Microglia control the glycinergic but not the GABAergic synapses via prostaglandin E2 in the spinal cord. The Journal of Cell Biology. jcb.201607048. doi:10.1083/jcb.201607048

Béchade C, S. Colasse, M. Diana, M. Rouault, and A. Bessis (2014) NOS2 expression is restricted to neurons in the healthy brain but is triggered in microglia upon inflammation. Glia. 62:956–63. doi:10.1002/glia.22652

Squarzoni P, G. Oller, G. Hoeffel, L. Pont-Lezica, P. Rostaing, D. Low, A. Bessis, F. Ginhoux, and S. Garel (2014) Microglia modulate wiring of the embryonic forebrain. CellReports. 8:1271-9. doi:10.1016/j.celrep. 2014.07.042

Pont-Lezica L, W. Beumer, S. Colasse, H. Drexhage, M. Versnel, and A. Bessis (2014) Microglia shape corpus callosum axon tract fasciculation : functional impact of prenatal inflammation. Eur J Neurosci. 39:1551–7. doi:10.1111/ejn.12508

Pascual O, S.B. Achour, P. Rostaing, A. Triller, and A. Bessis (2012) Microglia activation triggers astrocyte-mediated modulation of excitatory neurotransmission. Proc Natl Acad Sci USA. 109:E197–205. doi:10.1073/ pnas.1111098109

Fundamental mechanisms and human-specific regulations of synaptic development and plasticity

Andrea Dumoulin (équipe Triller) — 2015-02-11T13:56:52Z

Head of Project : Cécile Charrier

Synapses are multimolecular nanomachines that ensure the proper connectivity of neuronal circuits, integrate diverse inputs into biochemical reactions and allow adaptive responses to the environment. Small changes in the molecular organization of synapses can lead to profound modifications of behavioral and cognitive abilities, as frequently observed in neurodevelopmental and psychiatric disorders. Small changes also appeared during human evolution. Although poorly characterized, they underlie the distinctive developmental and physiological properties of human synapses and are at the core of what makes us humans.

We are investigating the role of molecular pathways linked to human evolution in the development and plasticity of synaptic connections in the neocortex. Our goal is to elucidate fundamental principles that are common to all mammals, and uncover regulations that are specific to humans. We employ a multidisciplinary approach in mouse and human models based on sparse in vivo manipulations in intact cortical circuits, proteomics, electrophysiology, as well as confocal, super-resolutive, and correlative microscopy.

Our recent work has focused on SRGAP2, one of the few genes specifically duplicated in the human lineage. We have demonstrated that the human-specific copy of SRGAP2 inhibits the ancestral protein, which leads to the emergence of human features of synapses such as their protracted developmental period and their increased density along dendrites. By investigating the underlying molecular basis, we have unraveled an intrinsic mechanism coordinating the development of excitatory and inhibitory synapses.

Participants : C. Charrier, M. Fossati, N. Assendorp, O. Gemin, S. Colasse and P. Rostaing.

Funding : ANR, Fyssen Foundation

Maffei A, Charrier C, Caiati MD, Barberis A, Mahadevan V, Woodin MA, Tyagarajan SK (2017) Emerging Mechanisms Underlying Dynamics of GABAergic Synapses. J Neurosci. 37:10792-10799

Fossati M, Pizzarelli R, Schmidt ER, Kupferman JV, Stroebel D, Polleux F, Charrier C (2016) SRGAP2 and Its Human-Specific Paralog Co-Regulate the Development of Excitatory and Inhibitory Synapses. Neuron 91:356-69

Charrier C, Polleux F (2012) [How human-specific SRGAP2 gene duplications control human brain development]. Med Sci (Paris) 28:911-4

Charrier C, Joshi K, Coutinho-Budd J, Kim JE, Lambert N, de Marchena J, Jin WL, Vanderhaeghen P, Ghosh A, Sassa T, Polleux F (2012) Inhibition of SRGAP2 function by its human-specific paralogs induces neoteny during spine maturation. Cell 149:923-35

Synaptic microstructure, dynamics and molecular interactions

Andrea Dumoulin (équipe Triller) — 2015-02-11T11:40:21Z

Three directions are considered here, and through their combination we aim at a deeper understanding of the molecular dynamics at synapses.

1- The transmission of signals between neurons is a function of the molecular organisation of synapses. In other words, the function of a synapse is determined by the number, activity and precise location of its components. In order to explore the ultrastructure of excitatory and inhibitory synapses we use advanced electron microscopy techniques such as high pressure freezing (HPF) and correlative light and electron microscopy (CLEM). In addition, we have implemented photo-activated localisation microscopy (PALM) to elucidate the absolute numbers and distribution of synaptic proteins at super-resolution scale.

Participants : A. Triller, P. Rostaing, F. Niwa, A. Ludwig, P. Serna Martinez, O. Gemin

2- Functional changes at synapses are necessarily the result of dynamic rearrangements of the synaptic structure. Over the last decade our laboratory has investigated the diffusion properties of inhibitory neurotransmitter receptors using single particle tracking (SPT) of quantum dot (QD) tagged receptor subunits at high spatial and temporal resolution. These studies have revealed the dynamic exchange of receptors between the synaptic and the extra-synaptic membrane as well as their immobilisation at synapses. More recently, we have begun to investigate the molecular interactions between receptors and scaffold proteins at synapses using high-density PALM-based single molecule imaging.

Participants : C. Specht, A. Triller, X. Yang, A. Jan, S. Colasse, P. Rostaing

3- Dynamic rearrangements of the synaptic structure are the basis of synaptic plasticity. Using pharmacological and physiological approaches as well as recombinant probes, we explore the upstream signalling processes that regulate the structure and function of synapses in cultured neurons. This includes changes in synaptic activity, activation of signalling molecules and post-translational modifications, the role of the extracellular matrix (ECM) and the presence of diffusion barriers for the dynamic exchange of synaptic components.

Participants : A. Dumoulin, A. Triller, V. Lepetz, P. Rostaing, S. Colasse

Patrizio A, Renner M, Pizzarelli R, Triller A, Specht CG (2017) Alpha subunit-dependent GlyR clustering and regulation of synaptic occupancy. Scientific Reports In press

Renner M, Wang L, Levi S, Hennekinne L, Triller A (2017) A simple and powerful analysis of lateral subdiffusion using single particle tracking. Biophys J in press

Shrivastava AN, Aperia A, Melki R, Triller A (2017) Physico-Pathologic Mechanisms involved in Neurodegeneration : Misfolded Proteins-Plasma Membrane Interactions. Neuron 95(1):33-50 ; doi : 10.1016

Bannai H, Niwa F, Sherwood MW, Shrivastava AN, Arizono M, Miyamoto A, Sugiura K, Lévi S, Triller A, Mikoshiba K (2015) Bidirectional Control of Synaptic GABAAR Clustering by Glutamate and Calcium. Cell Report 13:2768-80

Shrivastava AN, Redeker V, Fritz N, Pieri L, Almeida LG, Spolidoro M, Liebmann T, Bousset L, Renner M, Léna C, Aperia A, Melki R, Triller A (2015) α-synuclein assemblies sequester neuronal 3-Na+/K+-ATPase and impair Na+ gradient. EMBO J 34:2408-23

Specht CG, Izeddin I, Rodriguez PC, El Beheiry M, Rostaing P, Darzacq X, Dahan M, Triller A (2013) Quantitative nanoscopy of inhibitory synapses : counting gephyrin molecules and receptor binding sites. Neuron 79 : 308-321

Publications

Andrea Dumoulin (équipe Triller), Sarah Reemers — 2015-02-10T13:55:08Z

2017

Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ. (2017) Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses. Proc Natl Acad Sci U S A 114:11763-11768

Patrizio A, Renner M, Pizzarelli R, Triller A, Specht CG (2017) Alpha subunit-dependent GlyR clustering and regulation of synaptic occupancy. Scientific Reports In press

Renner M, Wang L, Levi S, Hennekinne L, Triller A (2017) A simple and powerful analysis of lateral subdiffusion using single particle tracking. Biophys J in press

Cantaut-Belarif Y, Antri M, Pizzarelli R, ColasseS, Vaccari I, Soares S, Renner M, Dallel R, Triller A*, Bessis B* (2017) Microglia control the glycinergic but not the GABAergic synapses via prostaglandin E2 in the spinal cord J Cell Biol 216:2979-2989

Ranft J, Almeida LG , Rodriguez PC , Triller A*, HakimV* (2017) An aggregation-removal model for the formation and size determination of post-synaptic scaffold domains. PloS Comput Biology Biol. 2017 Apr 24 ;13(4):e1005516

Shrivastava AN, Aperia A, Melki R, Triller A (2017) Physico-Pathologic Mechanisms involved in Neurodegeneration : Misfolded Proteins-Plasma Membrane Interactions. Neuron 95(1):33-50 ; doi : 10.1016

Maffei A, Charrier C, Caiati MD, Barberis A, Mahadevan V, Woodin MA, Tyagarajan SK (2017) Emerging Mechanisms Underlying Dynamics of GABAergic Synapses. J Neurosci. 37:10792-10799

2016

Hannan S, Gerrow K, Triller A, Smart TG (2016) Phospho-dependent Accumulation of GABABRs at Presynaptic Terminals after NMDAR Activation. Cell Rep. 16:1962-73

Shrivastava AN, Redeker V, Fritz N, Pieri L, Almeida LG, Spolidoro M, Liebmann T, Bousset L, Renner M, Léna C, Aperia A, Melki R, Triller A (2016) Data in support of the identification of neuronal and astrocyte proteins interacting with extracellularly applied oligomeric and fibrillar synuclein assemblies by mass spectrometry. Data in Brief 7 : 221-8

El Beheiry M, T¨urkcan S, Richly M, Triller A, Alexandrou A, Dahan M & Masson J.-B. (2016) A Primer on the Bayesian Approach to High-density Single-Molecule Trajectories Analysis. Biophysical J 110:1209-15

Wang I, Dumoulin A, Renner M, Triller A*, Specht CG (2016) The Role of Synaptopodin in Membrane Protein Diffusion in the Dendritic Spine Neck PLoS ONE 11(2) : 1-18 e0148310. doi:10.1371/

2015

Plamont MA, Billon-Denis E, Maurin S, Gauron C, Pimenta FM, Specht CG, Shi J, Quérard J, Pan B, Rossignol J, Morellet N, Volovitch M, Lescop E, Chen Y, Triller A, Vriz S, Le Saux T, Jullien L, Gautier A (2015) A small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo. Proc Natl Acad Sci USA 113:497-502

Haselwandter CA, Kardar M, Triller A, da Silveira RA (2015) Self-assembly and plasticity of synaptic domains through a reaction-diffusion mechanism. Phys Rev E Stat Nonlin Soft Matter Phys. 92(3-1):032705. Epub 2015 Sep 3

Hausrat T, Muhia M, Gerrow K, Thomas P, Hirdes W, Tsukita S, Heisler F, Herich L, Dubroqua S, Breiden P, Feldon J, Schwarz J, Yee B, Smart T, Triller A, Kneussel M (2015) Radixin regulates synaptic GABAA receptor density and is essential for reversal learning and short-term memory. Nature Com. doi:10.1038/ncomms7872

Nadjar Y, Triller A, Bessereau JL, Dumoulin A (2015) The Susd2 protein regulates neurite growth and excitatory synaptic density in hippocampal cultures. Mol Cell Neurosci. 65:82-91

Salvatico C, Specht CG, Triller A (2015) Synaptic receptor dynamics : from theoretical concepts to deep quantification and chemistry in cellulo. Neuropharmacology. 88:2-9

2014

Gouzer G, Specht CG, Allain L, Shinoe T, Triller A (2014) Benzodiazepine-dependent stabilization of GABA(A) receptors at synapses. Mol Cell Neurosci. 63:101-13

Kneussel M, Triller A, Choquet D (2014) SnapShot : receptor dynamics at plastic synapses.
Cell. 157(7):1738-1738.e1

Gerrow K, Triller A. (2014) GABAA receptor subunit composition and competition at synapses are tuned by GABAB receptor activity. Mol Cell Neurosci. 60:97-107

Masson JB, Dionne P, Salvatico C, Renner M, Specht CG, Triller A, Dahan M (2014) Mapping the energy and diffusion landscapes of membrane proteins at the cell surface using high-density single-molecule imaging and Bayesian inference : application to the multiscale dynamics of glycine receptors in the neuronal membrane. Biophys J 106 : 74-83

Horellou S, Pascual O, Triller A, Marty S (2014) Adaptive and non-adaptive changes in activity-deprived presynaptic terminals. Eur J Neurosci 39 : 61-71

2013

Siksou L, Silm K, Biesemann C, Nehring RB, Wojcik SM, Triller A, El Mestikawy S, Marty S, Herzog E (2013) A role for vesicular glutamate transporter 1 in synaptic vesicle clustering and mobility. Eur J Neurosci 37 : 1631-1642

Shrivastava AN, Rodriguez PC, Triller A, Renner M (2013) Dynamic micro-organization of P2X7 receptors revealed by PALM based single particle tracking. Front Cell Neurosci 7(232) : doi : 10.3389

Shrivastava AN, Kowalewski JM, Renner M, Bousset L, Koulakoff A, Melki R, Giaume C, Triller A, (2013) beta-amyloid and ATP-induced diffusional trapping of astrocyte and neuronal metabotropic glutamate type-5 receptors. Glia 61 : 1673-1686

Hennekinne L, Colasse S, Triller A, Renner M (2013) Differential control of thrombospondin over synaptic glycine and AMPA receptors in spinal cord neurons. J Neurosci 33 : 11432-11439

Choquet D, Triller A (2013) The dynamic synapse. Neuron 80 : 691-703

2012

Triller A, Sheng M (2012) Synaptic structure and function. Curr Opin Neurobiol 22 : 363-365

Renner M, Schweizer C, Bannai H, Triller A, Levi S (2012) Diffusion barriers constrain receptors at synapses. PLoS One 7 : e43032

Pascual O, Ben Achour S, Rostaing P, Triller A, Bessis A (2012) Microglia activation triggers astrocyte-mediated modulation of excitatory neurotransmission. Proc Natl Acad Sci U S A 109 : E197-E205

Niwa F, Bannai H, Arizono M, Fukatsu K, Triller A, Mikoshiba K (2012) Gephyrin-Independent GABA(A)R Mobility and Clustering during Plasticity. PLoS One 7 : doi/10.1371

Izeddin I, Boulanger J, Racine V, Specht CG, Kechkar A, Nair D, Triller A, Choquet D, Dahan M, Sibarita JB (2012) Wavelet analysis for single molecule localization microscopy. Opt Express 20 : 2081-2095

2011

Triller A (2011f) Molecular Imagery : towards in cellulo chemistry. MS-Med Sci 27 : 451-452

Specht CG, Grunewald N, Pascual O, Rostgaard N, Schwarz G, Triller A (2011) Regulation of glycine receptor diffusion properties and gephyrin interactions by protein kinase C. Embo J 30 : 3842-3853

Sillibourne JE, Specht CG, Izeddin I, Hurbain I, Tran P, Triller A, Darzacq X, Dahan M, Bornens M (2011) Assessing the localization of centrosomal proteins by PALM/STORM nanoscopy. Cytoskeleton 68) : 619-627

Siksou L, Triller A, Marty S (2011) Ultrastructural organization of presynaptic terminals. Curr Opin Neurobiol 21 : 261-268

Shrivastava AN, Triller A, Sieghart W, Sarto-Jackson I (2011) Regulation of GABA(A) Receptor Dynamics by Interaction with Purinergic P2X(2) Receptors. J Biol Chem 286 : 14455-14468

Shrivastava AN, Triller A, Sieghart W (2011) GABA(A) Receptors : Post-Synaptic Co-Localization and Cross-Talk with Other Receptors. Front Cell Neurosci 5 : doi : 10.3389/fncel.2011.00007

Ribrault C, Sekimoto K, Triller A (2011) From the stochasticity of molecular processes to the variability of synaptic transmission. Nature Rev Neurosci 12 : 375-387

Ribrault C, Reingruber J, Petkovic M, Galli T, Ziv NE, Holcman D, Triller A (2011) Syntaxin1A lateral diffusion reveals transient and local SNARE interactions. J Neurosci 31 : 17590-17602

Renner M, Domanov Y, Sandrin F, Izeddin I, Bassereau P, Triller A (2011) Lateral diffusion on tubular membranes : quantification of measurements bias. PLoS One 6 : e25731

Mukherjee J, Kretschmannova K, Gouzer G, Maric HM, Ramsden S, Tretter V, Harvey K, Davies PA, Triller A, Schindelin H, Moss SJ (2011) The Residence Time of GABA(A)Rs at Inhibitory Synapses Is Determined by Direct Binding of the Receptor alpha 1 Subunit to Gephyrin. J Neurosci 31 : 14677-14687

Machado P, Rostaing P, Guigonis JM, Renner M, Dumoulin A, Samson M, Vannier C, Triller A (2011) Heat Shock Cognate Protein 70 Regulates Gephyrin Clustering. J Neurosci 31 : 3-14

Levi S, Dahan M, Triller A (2011) Labeling neuronal membrane receptors with quantum dots. Cold Spring Harb Protoc 2011 (1) : prot5580

Izeddin I, Specht CG, Lelek M, Darzacq X, Triller A, Zimmer C, Dahan M (2011) Super-Resolution Dynamic Imaging of Dendritic Spines Using a Low-Affinity Photoconvertible Actin Probe. PLoS One 6 : e15611

Haselwandter CA, Calamai M, Kardar M, Triller A, da Silveira RA (2011) Formation and Stability of Synaptic Receptor Domains. Phys Rev Lett 106 : 238104

Domanov YA, Aimon S, Toombes GES, Renner M, Quemeneur F, Triller A, Turner MS, Bassereau P (2011) Mobility in geometrically confined membranes. Proc Natl Acad Sci U S A 108 : 12605-12610

Bechade C, Pascual O, Triller A, Bessis A (2011) Nitric oxide regulates astrocyte maturation in the hippocampus : Involvement of NOS2. Molec Cell Neurosci 46 : 762-769