IBENS - ENS

Remerciement

Fatima MELOUKI (microscopie photonique) — 2021-09-03T08:40:01Z

Le travail accompli au sein de la plateforme photonique de l'IBENS doit être reconnu. De ce fait, des remerciements sont de circonstances. Nous vous serions grés de citer la phrase ci dessous en cas d'utilisation d'images générées par nos microscopes ou de l'utilisation de nos logiciels d'analyse.

We gratefully acknowledge [platform staff name] and the IBENS imaging facility (IMACHEM-IBiSA), member of the national infrastructure France-BioImaging supported by the French National Research Agency (ANR-24-INBS-0005 FBI BIOGEN)", which received support from the "Fédération pour la Recherche sur le Cerveau - Rotary International France" (2025) , from the program « Investissements d'Avenir » ANR-10-LABX-54 MEMOLIFE and supported by the DIM C-BRAINS, funded by the Conseil Régional d'Ile-de-France

Microscope plein champs

Fatima MELOUKI (microscopie photonique) — 2021-07-29T10:59:45Z

Microscope plein champs Leica

Utilisations

Observation de tissus fixés et vivants
Acquisition 3D z-stack, time-lapse, spectral scan
Acquisition multi-position et mosaïque
Fluorophore observable : DAPI, GFP, CY5 et CY3

Spécifications

Microscope plein champs DMi6000
Platine motorisée XY
Platine Z motorisée
Logiciel : Inscopper
Détecteurs : caméra iris 15 (25 mm detecteur, taille de pixel 4.25 micrometres)
Objectifs : 10x, 40x, 63x, 100x
Lasers : lampe fluo LEICA EL6000

Tarifs

Fatima MELOUKI (microscopie photonique) — 2021-02-12T14:08:15Z

Les samedis et dimanche ainsi que lors des heures creuses, les tarifs affichés ci-dessus sont réduis de 50%.
Les utilisateurs externe doivent prépayer les heures à effectuer en effectuant un bon de commande correspondant à un montant minimum de 300 euros.

LightSheet microscope

Fatima MELOUKI (microscopie photonique) — 2018-11-13T11:38:41Z

LightSheet

La plateforme met à disposition 1 microscope lightsheet :

Utilisation

Observation de tissus clarifié
Protocole : idisco & disco + (DBE) + cubic
Acquisition 3D z-stack
Acquisition en mosaïques

Spécifications

Ultramicroscope II
Détecteurs : sCMOS Andor Neo ( 2560 x 2160 pixels, Taille d'un pixel = 6.5 x 6.5 µm )
Objectif : 2x
Zoom possible : 1.26x - 12.6x
Lasers : 488nm, 561nm, 640nm, 785nm.

Microscopes Spinning-disk

Fatima MELOUKI (microscopie photonique) — 2018-11-13T11:38:36Z

La plateforme met à disposition 2 microscopes spinning disk :

Spinning Disk Inversé

Utilisations

Observation de tissus vivants
Acquisition 3D z-stack, time-lapse
Acquisition multi-position
Filtres : DAPI, Cy3, Cy5, GFP
Chambre thermostatée + régulation du CO2

Spécifications

Spinning-disk CSUX1-A1 Yokogawa
Microscope Nikon Ti avec PFS
Platine motorisée XY
Platine Z motorisée piezo 100 µm
Logiciel : METAMORPH Premier 7.6
Détecteur : Caméra Hamamatssu Orca flash4.0 (Type : CMOS ; Taille d'un pixel = 6.5 x 6.5 µm ; rendement quantique = 82%)
Objectifs : 10x air, 40x à eau, 63x et 100x à huile
Lasers : 405, 491 nm ,561 nm , 640nm

Spinning Disk Inversé SORA

Utilisation

Observation de tissus vivants
Acquisition 3D z-stack, time-lapse
Acquisition multi-position
Fluorophore observable : du DAPI au Cy3
Chambre thermostatée + régulation du CO2

Spécifications

Spinning-disk CSUW1- Yokogawa
Microscope Nikon Ti PFS
Platine motorisée XY
Platine Z motorisée piezo 100 µm
Logiciel : NIS
Détecteur : Caméra Kinetic (Type : CMOS ; Taille d'un pixel = 6.5 x 6.5 µm ; rendement quantique > 95 %)
Objectifs : 10x air, 40x à eau, 63x et 100x à huile
Lasers : 405nm, 488nm, 561, 633nm
Module SORA => Gain de résolution

Microscope Confocal

Fatima MELOUKI (microscopie photonique) — 2018-11-13T11:38:34Z

Confocal Leica TCS SP8

Utilisations

Observation de tissus fixés et vivants
Acquisition 3D z-stack, time-lapse, spectral scan
Acquisition multi-position et mosaïque
Filtres disponibles : du GFP au Cy5

Configuration

Microscope Leica DMI6000
Platine motorisé XY
Logiciel : LAS X
Détecteurs : 1 photomultiplicateur (PMT) + 2 Hybrides (HyD) + 1 PMT en transmission
Objectifs : 10x air, 20 et 25 multi-immersion, 40x à eau, 40x et 63x à huile
Lasers : Laser 405nm + Laser blanc accordable de 470 à 670 nm

Confocal Nikon Ax

Fonctionnalité

Ce microscope est dédié aux échantillons transparisés car équipé d'objectif à faible grossissement, et de grande distance de travail.Il permet d'une part l'acquisition de Z-stack, time-lapse, spectral scan et d'autre part de réaliser du multi-position et des mosaïques avec une platine XYZ motorisée

Configuration

Objectifs : Nikon 16x/ON 0.8 / distance de travail 3 mm / Immersion eau
Cubes filters : DAPI, GFP, CY3
Lasers : LUA-S4 Laser Unit 405 / 488 / 561 / 640 nm
Détecteurs : 2 détecteurs GaAsp (détection simultanée et séquentielle)

Logiciel : NIS Elements C

Confocal Nikon Ax NSparc

Utilisations

Observation de tissus fixés et vivants
Acquisition 3D z-stack et time-lapse
Acquisition multi-position et mosaïque
Fluorophore observable : du DAPI au Cy5

Spécifications

Microscope Nikon TiE2
Platine motorisée XY
Platine Z motorisée + Sur-platine résonnant
Logiciel : NIS
Chambre thermostatée
Détecteurs : 3 detecteurs GaAsp + detecteurs NSparc
Objectifs : 10x air, 40x huile, 60x huile, 100x
Lasers : 405 nm, 488nm, 561nm, 633nm

Karthala AODscope multiphoton

Fatima MELOUKI (microscopie photonique) — 2017-04-27T08:33:36Z

more info : karthala system

Detection :

Dodt contraste with video rate
4 PMT GaAsP photon counting or analog (2 epifluorescence and 2transfluorescence)
Light guide epi detection
High efficiency design detection

Scanning :

Two AOD tunable design with temporal and spatial compensation laser lines ( 700-900nm and 800-1000nm). they can work simultaneously or separate.

Mode Random-Access scanning :
This allows to increase the dwell-time per point and dramatically improves the resolution. 50Khz/nbr point .( Otsu et al. paper Neuron 2014)

Mode fast image scanning :
Capacity to scan at adjustable speed from 0.1µs/pixel (resonant speed) to any dwell time, this spanning the range between resonant scanners and classical scanners. Contrary to resonant scanners the scan is linear and sub-fields can be scanned at resonant speed independently.it's also possible to make multi region of interest.The field of view is 350 µm.

Stabilisation of sample :
Automatic 3D stabilisation of episodic acquisitions (during the inter-episode dwell time, ie in between 2 repetitive stimulations for example)

AODscope software :

Karthala imaging software

Associate Paper :

‘Ultrafast random-access scanning in twophoton microscopy using acousto-optic deflectors.'
Salome´ R et al J Neurosci Methods 2006

‘A spatio-temporally compensated acousto-optic scanner for two-photon microscopy providing large field of view.'
Kremer Y et al Opt Express 2008

‘Optical monitoring of neuronal activity at high frame rate with a digital random-access multiphoton (RAMP)'
Otsu Y et al J Neurosci Methods 2008

‘Activity-Dependent Gating of Calcium Spikes by A-type K+ Channels Controls Climbing fiber signaling in purkinje cell dentrires.'
Ostsu Y et al , Neuron 2014

‘Interplay between Synchronization of Multivesicular Release and Recruitment of Additional Release Sites Support Short-Term Facilitation at Hippocampal Mossy Fiber to CA3 Pyramidal Cells Synapses.'
Chamberland et al Jneurosci 2014

‘Burst-Dependent Bidirectional Plasticity in the Cerebellum Is Driven by Presynaptic NMDA Receptors'
Bouvier et al Cell Reports 2016

‘Diversity in cell motility reveals the dynamic nature of the formation of zebrafish taste sensory organs'
Soulika et al Development 2016

‘Reallocation of Olfactory Cajal-Retzius Cells Shapes Neocortex Architecture'
Frutos et al Neuron 2016

Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators
Chamberland et al eLIFE 2017

Action potential counting at giant mossy fiber terminals gates information transfer in the hippocampus
Chamberland et al PNAS 2018

Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties
Hernandez et al Scientific Reports 2018

Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice
Vincent Villette, Mariya Chavarha, Ivan K. Dimov, ..., Jun Ding, Stephane Dieudonne, Michael Z. Lin, Cell 2019.

Station de traitement et d'analyse d'images

Fatima MELOUKI (microscopie photonique) — 2016-12-02T16:49:14Z

Arivis : Logiciel de reconstruction et d'analyse d'image multidimensionelles adapté pour les fichiers de grandes tailles (>20 Go).

Il permet de visualiser des acquisition 3D, mosaïques d'images, exporter sous forme d'images ou de film avec modélisation 3D .

Imaris : Logiciel propriétaire de reconstruction 3D/4D, édité par la société Bitplane. Il permet de visualiser, les images prises en microscopie photonique, par reconstruction volumique et surfacique.

Les options disponibles en plus permettent de suivre des objets dans le temps et l'espace pour obtenir différentes informations sous forme de tableur (nombre d'objets, surfaces, volumes, distances, coordonnées, trajectoires, vitesses...).

Publication

Fatima MELOUKI (microscopie photonique) — 2015-04-22T11:32:24Z

2026

Microglial colonization of the developing mouse brain is controlled by both microglial and neural CSF-1.
Bridlance C, Viguier S, Olivié N, Dupont E, Thobois D, Mathieu B, Jiang JX, López Bendito G, Greter M, Becher B, Ginhoux F, Silvin A, Klingler E, Garel S, Thion MS
EMBO J. 2026 Jan. 45(1):151-181.

2025

Label-free nonlinear microscopy probes cellular metabolism and myelin dynamics in live tissue.
Asadipour B, Morizet J, Ronzano R, Zhang X, Aigrot MS, Mahou P, Solinas X, Phan MS, Chessel A, Stankoff B, Desmazieres A, Beaurepaire E, Stringari C
Commun Biol. 2025 Nov 23. 8(1):1811

mTOR controls ependymal cell differentiation by targeting the alternative cell cycle and centrosomal proteins.
Bankolé A, Srivastava A, Shihavuddin A, Tighanimine K, Faucourt M, Koka V, Weill S, Nemazanyy I, Nelson AJ, Stokes MP, Delgehyr N, Genovesio A, Meunier A, Fumagalli S, Pende M, Spassky N
EMBO Rep. 2025 Jun. 26(12):3075-3105.

A genetically defined pontine nucleus essential for ingestion in mice.
Sungeelee S, Mailhes Hamon C, Chettouh Z, Bokiniec P, Eymael A, McMullan S, Léna C, Dempsey B, Brunet JF Proc Natl Acad Sci U S A. 2025 Jul 22. 122(29):e2411174122.

Identification and Organization of a Postural Anti-Gravity Module in the Cerebellar Vermis.
Gouhier A, Villette V, Mathieu B, Ayon A, Bradley J, Dieudonné S Neuroscience. 2025 Jul 10. 578:33-45

Unequal mitochondrial segregation promotes asymmetric fates during neurogenesis. Bunel B, Leclercq R, Goïame R, Gautier A, Morin X, Fischer E. Nat Commun. 2025 Dec 15 ;16(1):11049. doi : 10.1038/s41467-025-66932-0. PMID : 41398154 ; PMCID : PMC12706016.

Hansen, Enrique Carlos Arnoldo, Nicole Sanderson, Sarah Nourin, Virginie Candat, Carina Curto, and German Sumbre. (2025). 'Topological Analysis of Neuronal Assemblies Reveals Low-Rank Structure Modulated by Cholinergic Activity'. bioRxiv

Janbon, Maxime, Mateo Amortegui, Enrique Carlos Arnoldo Hansen, Sarah Nourin, Germán Sumbre, and Adrián Ponce-Alvarez. (2025) 'Critical Neuronal Avalanches Emerge from Excitation-Inhibition Balanced Spontaneous Activity'. bioRxiv

2024

A novel rhodopsin-based voltage indicator for simultaneous two-photon optical recording with GCaMP in vivo. Villette, V., Yang, S., Valenti, R., Macklin, J. J., Bradley, J., Mathieu, B., Lombardini, A., Podgorski, K., Dieudonné, S., Schreiter, E. R., & Abdelfattah, A. S. (2024). bioRxiv : the preprint server for biology, 2024.11.15.623698. https://doi.org/10.1101/2024.11.15.623698

Identification and Organization of a Postural Anti-Gravity Module in the Cerebellar Vermis.
Gouhier A, Villette V, Mathieu B, Ayon A, Bradley J, Dieudonné S
Neuroscience. 2024 Jun 17. https://doi.org/10.1016/j.neuroscience.2024.06.006

Neurophotonics beyond the surface : unmasking the brain's complexity exploiting optical scattering.
Xia F, Rimoli CV, Akemann W, Ventalon C, Bourdieu L, Gigan S, de Aguiar HB
Neurophotonics. 2024 Sep. 11(Suppl 1):S11510. https://doi.org/10.1117/1.NPh.11.S1.S11510

Acousto-optic holography for pseudo-two-dimensional dynamic light patterning.
Akemann W, Bourdieu L
APL Photonics. 2024 Apr 1. 9(4):046103. https://doi.org/10.1063/5.0185857

Neurophotonics beyond the Surface : Unmasking the Brain's Complexity Exploiting Optical Scattering.
Xia F, Rimoli CV, Akemann W, Ventalon C, Bourdieu L, Gigan S, de Aguiar HB
ArXiv. 2024 Mar 21. https://www.ncbi.nlm.nih.gov/pubmed/38562443

Microglia maintain structural integrity during fetal brain morphogenesis.
Lawrence AR, Canzi A, Bridlance C, Olivié N, Lansonneur C, Catale C, Pizzamiglio L, Kloeckner B, Silvin A, Munro DAD, Fortoul A, Boido D, Zehani F, Cartonnet H, Viguier S, Oller G, Squarzoni P, Candat A, Helft J, Allet C, Watrin F, Manent JB, Paoletti P, Thieffry D, Cantini L, Pridans C, Priller J, Gélot A, Giacobini P, Ciobanu L, Ginhoux F, Thion MS, Lokmane L, Garel S
Cell. 2024 Feb 15. 187(4):962-980.e19. https://doi.org/10.1016/j.cell.2024.01.012

2023

Neurotransmitter content heterogeneity within an interneuron class shapes inhibitory transmission at a central synapse.
Dumontier D, Mailhes-Hamon C, Supplisson S, Dieudonné S.
Front Cell Neurosci. 2023 Jan 4 ;16:1060189. doi : 10.3389/fncel.2022.1060189. eCollection 2022.

The cerebellum regulates fear extinction through thalamo-prefrontal cortex interactions in male mice.
Frontera JL, Sala RW, Georgescu IA, Baba Aissa H, d'Almeida MN, Popa D, Léna C
Nat Commun. 2023 Mar 17. 14(1):1508. The cerebellum regulates fear extinction through thalamo-prefrontal cortex interactions in male mice. https://doi.org/10.1038/s41467-023-36943-w

Plasticity of thalamocortical axons is regulated by serotonin levels modulated by preterm birth.
Sinclair‑Wilson A, Lawrence A, Ferezou I, Cartonnet H, Mailhes C, Garel S, Lokmane L
Proc Natl Acad Sci U S A. 2023 Aug 15. 120(33):e2301644120. https://doi.org/10.1073/pnas.2301644120

Blochet B, Akemann W, Gigan S, Bourdieu L. Fast wavefront shaping for two-photon brain imaging with multipatch correction. Proc Natl Acad Sci U S A. 2023 Dec 19 ;120(51):e2305593120. doi : 10.1073/pnas.2305593120. Epub 2023 Dec 15. PMID : 38100413 ; PMCID : PMC10743372. https://doi.org/10.1073/pnas.2305593120

Neural tube-associated boundary caps are a major source of mural cells in the skin
Gerschenfeld G, Coulpier F, Gresset A, Pulh P, Job B, Topilko T, Siegenthaler J, Kastriti ME, Brunet I, Charnay P, Topilko P Elife. 2023 Dec 14. 12. https://doi.org/10.7554/eLife.69413

2022
Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy.
Liu Z, Lu X, Villette V, Gou Y, Colbert KL, Lai S, Guan S, Land MA, Lee J, Assefa T, Zollinger DR, Korympidou MM, Vlasits AL, Pang MM, Su S, Cai C, Froudarakis E, Zhou N, Patel SS, Smith CL, Ayon A, Bizouard P, Bradley J, Franke K, Clandinin TR, Giovannucci A, Tolias AS, Reimer J, Dieudonné S, St Pierre F
Cell. 2022 Sep 1. 185(18):3408-3425.e29.

GluN3A excitatory glycine receptors control adult cortical and amygdalar circuits.
Bossi S, Dhanasobhon D, Ellis‑Davies GCR, Frontera J, de Brito Van Velze M, Lourenço J, Murillo A, Luján R, Casado M, Perez‑Otaño I, Bacci A, Popa D, Paoletti P, Rebola N

Neuron. 2022 Aug 3. 110(15):2438-2454.e8.
Cerebellar stimulation prevents Levodopa-induced dyskinesia in mice and normalizes activity in a motor network.
Coutant B, Frontera JL, Perrin E, Combes A, Tarpin T, Menardy F, Mailhes Hamon C, Perez S, Degos B, Venance L, Léna C, Popa D
Nat Commun. 2022 Jun 9. 13(1):3211.

Dynamic interplay between thalamic activity and Cajal-Retzius cells regulates the wiring of cortical layer 1.
Genescu I, Aníbal Martínez M, Kouskoff V, Chenouard N, Mailhes Hamon C, Cartonnet H, Lokmane L, Rijli FM, López Bendito G, Gambino F, Garel S
Cell Rep. 2022 Apr 12. 39(2):110667.

2021

Fast optical recording of neuronal activity by three-dimensional custom-access serial holography.
Akemann W, Wolf S, Villette V, Mathieu B, Tangara A, Fodor J, Ventalon C, Léger JF, Dieudonné S, Bourdieu L Nat Methods. Epub 2021 Dec 23.

Maynard S, Rostaing P, Schaefer N, Gemin O, Candat A, Dumoulin A, Villmann C, Triller A, Specht CG.
Identification of a stereotypic molecular arrangement of endogenous glycine receptors at spinal cord synapses.
Elife. 2021 Dec 8 ;10 : e74441.

Gemin O, Serna P, Zamith J, Assendorp N, Fossati M, Rostaing P, Triller A, Charrier C.
Unique properties of dually innervated dendritic spines in pyramidal neurons of the somatosensory cortex uncovered by 3D correlative light and electron microscopy.
PLoS Biol. 2021 Aug 24 ;19(8) : e3001375

Gerschenfeld G, Aid R, Simon Yarza T, Lanouar S, Charnay P, Letourneur D, Topilko P
Tuning Physicochemical Properties of a Macroporous Polysaccharide-Based Scaffold for 3D Neuronal Culture.
Int J Mol Sci. 2021 Nov 25. 22(23).

Dempsey B, Sungeelee S, Bokiniec P, Chettouh Z, Diem S, Autran S, Harrell ER, Poulet JFA, Birchmeier C, Carey H, Genovesio A, McMullan S, Goridis C, Fortin G, Brunet JF
A medullary centre for lapping in mice.
Nat Commun. 2021 Nov 2. 12(1):6307.

2020

2019

Activity-dependent death of transient Cajal-Retzius neurons is required for functional cortical wiring.
Riva M, Genescu I, Habermacher C, Orduz D, Ledonne F, Rijli FM, López‑Bendito G, Coppola E, Garel S, Angulo MC, Pierani A
Elife. 2019 Dec 31. 8.

Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice.
Villette V, Chavarha M, Dimov IK, Bradley J, Pradhan L, Mathieu B, Evans SW, Chamberland S, Shi D, Yang R, Kim BB, Ayon A, Jalil A, St‑Pierre F, Schnitzer MJ, Bi G, Toth K, Ding J, Dieudonné S, Lin MZ
Cell. 2019 Dec 12. 179(7):1590-1608.e23.

Sensorimotor Transformations in the Zebrafish Auditory System.
Privat M, Romano SA, Pietri T, Jouary A, Boulanger‑Weill J, Elbaz N, Duchemin A, Soares D, Sumbre G
Curr Biol. 2019 Dec 2. 29(23):4010-4023.e4.

Trans-Synaptic Signaling through the Glutamate Receptor Delta-1 Mediates Inhibitory Synapse Formation in Cortical Pyramidal Neurons.
Fossati M, Assendorp N, Gemin O, Colasse S, Dingli F, Arras G, Loew D, Charrier C
Neuron. 2019 Dec 18. 104(6):1081-1094.e7.

Control of aversion by glycine-gated GluN1/GluN3A NMDA receptors in the adult medial habenula.
Otsu Y, Darcq E, Pietrajtis K, Mátyás F, Schwartz E, Bessaih T, Abi Gerges S, Rousseau CV, Grand T, Dieudonné S, Paoletti P, Acsády L, Agulhon C, Kieffer BL, Diana MA
Science. 2019 Oct 11. 366(6462):250-254.

Dynamics of centriole amplification in centrosome-depleted brain multiciliated progenitors.
Mercey O, Al Jord A, Rostaing P, Mahuzier A, Fortoul A, Boudjema AR, Faucourt M, Spassky N, Meunier A
Sci Rep. 2019 Sep 10. 9(1):13060.

Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members.
Ortiz‑Álvarez G, Daclin M, Shihavuddin A, Lansade P, Fortoul A, Faucourt M, Clavreul S, Lalioti ME, Taraviras S, Hippenmeyer S, Livet J, Meunier A, Genovesio A, Spassky N
Neuron. 2019 Apr 3. 102(1):159-172.e7.

Functional Alteration of Cerebello-Cerebral Coupling in an Experimental Mouse Model of Parkinson's Disease.
Menardy F, Varani AP, Combes A, Léna C, Popa D
Cereb Cortex. 2019 Apr 1. 29(4):1752-1766.

Clustering of Tau fibrils impairs the synaptic composition of α3-Na+/K+-ATPase and AMPA receptors.
Shrivastava AN, Redeker V, Pieri L, Bousset L, Renner M, Madiona K, Mailhes‑Hamon C, Coens A, Buée L, Hantraye P, Triller A, Melki R
EMBO J. 2019 Feb 1. 38(3).

Cellular Origin, Tumor Progression, and Pathogenic Mechanisms of Cutaneous Neurofibromas Revealed by Mice with Nf1 Knockout in Boundary Cap Cells.
Radomska KJ, Coulpier F, Gresset A, Schmitt A, Debbiche A, Lemoine S, Wolkenstein P, Vallat JM, Charnay P, Topilko P
Cancer Discov. 2019 Jan. 9(1):130-147.

2018

Active intermixing of indirect and direct neurons builds the striatal mosaic.
Tinterri A, Menardy F, Diana MA, Lokmane L, Keita M, Coulpier F, Lemoine S, Mailhes C, Mathieu B, Merchan‑Sala P, Campbell K, Gyory I, Grosschedl R, Popa D, Garel S
Nat Commun. 2018 Nov 9. 9(1):4725.

Microglia and early brain development : An intimate journey.
Thion MS, Ginhoux F, Garel S
Science. 2018 Oct 12. 362(6411):185-189.

Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties.
Hernandez O, Pietrajtis K, Mathieu B, Dieudonné S
Sci Rep. 2018 Sep 13. 8(1):13768.

Ependymal cilia beating induces an actin network to protect centrioles against shear stress.
Mahuzier A, Shihavuddin A, Fournier C, Lansade P, Faucourt M, Menezes N, Meunier A, Garfa‑Traoré M, Carlier MF, Voituriez R, Genovesio A, Spassky N, Delgehyr N
Nat Commun. 2018 Jun 11. 9(1):2279.

Tangential migration of corridor guidepost neurons contributes to anxiety circuits.
Tinterri A, Deck M, Keita M, Mailhes C, Rubin AN, Kessaris N, Lokmane L, Bielle F, Garel S
J Comp Neurol. 2018 Feb 15. 526(3):397-411.

The "sacral parasympathetic" : ontogeny and anatomy of a myth.
Espinosa‑Medina I, Saha O, Boismoreau F, Brunet JF
Clin Auton Res. 2018 Feb. 28(1):13-21.

Functional Principles of Posterior Septal Inputs to the Medial Habenula.
Otsu Y, Lecca S, Pietrajtis K, Rousseau CV, Marcaggi P, Dugué GP, Mailhes‑Hamon C, Mameli M, Diana MA
Cell Rep. 2018 Jan 16. 22(3):693-705.

2017
Calibrated mitotic oscillator drives motile ciliogenesis
Adel Al Jord, Asm Shihavuddin, Raphaël Servignat d'Aout, Marion Faucourt, Auguste Genovesio, Anthi Karaiskou, Joëlle Sobczak-Thépot, Nathalie Spassky, Alice Meunier.
Science. 2017 Oct 5. pii : eaan8311. doi : 10.1126/science.aan8311

Dual origin of enteric neurons in vagal Schwann cell precursors and the sympathetic neural crest
Isabel Espinosa-Medina, Ben Jevans, Franck Boismoreau, Zoubida Chettouh, Hideki Enomoto, Thomas Müller, Carmen Birchmeier, Alan J. Burns, and Jean-François Brunet. doi : 10.1073/pnas.1710308114

Endodermal germ-layer formation through active actin-driven migration triggered by N-cadherin.
Giger FA, David NB
Proc Natl Acad Sci U S A. 2017 Sep 19. 114(38):10143-10148.

Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner
Morgane Sonia Thion, Donovan Low, Aymeric Silvin, Jinmiao Chen, Pauline Grisel, Jonas Schulte-Schrepping, Ronnie Blecher, Thomas Ulas, Paola Squarzoni, Guillaume Hoeffel, Fanny Coulpier, [...] Sven Pettersson, Florent Ginhoux, Sonia Garel.
doi.org/10.1016/j.cell.2017.11.042

mTORC1 signaling and primary cilia are required for brain ventricle morphogenesis.
Foerster P, Daclin M, Asm S, Faucourt M, Boletta A, Genovesio A, Spassky N
Development. 2017 Jan 15. 144(2):201-210.

Microglia control the glycinergic but not the GABAergic synapses via prostaglandin E2 in the spinal cord.
Cantaut‑Belarif Y, Antri M, Pizzarelli R, Colasse S, Vaccari I, Soares S, Renner M, Dallel R, Triller A, Bessis A
J Cell Biol. 2017 Sep 4. 216(9):2979-2989.

Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers.
Patrizio A, Renner M, Pizzarelli R, Triller A, Specht CG
Sci Rep. 2017 Sep 7. 7(1):10899.

2016

Cristina A. de Frutos, Guy Bouvier, Yoko Arai, Morgane S. Thion, Ludmilla Lokmane, Maryama Keita, Mario Garcia-Dominguez, Patrick Charnay, Tatsumi Hirata, Dieter Riethmacher, Elizabeth A. Grove, Fadel Tissir, Mariano Casado, Alessandra Pierani, and Sonia Garel Reallocation of Olfactory Cajal-Retzius
Cells Shapes Neocortex Architecture Neuron

Matteo Fossati, Rocco Pizzarelli, Ewoud R. Schmidt, Justine V. Kupferman, David Stroebel, Franck Polleux, Cecile Charrier. SRGAP2 and Its Human-Specific Paralog Co-Regulate the Development of Excitatory and Inhibitory Synapses Neuron

I. Espinosa-Medina, O. Saha, F. Boismoreau, Z. Chettouh, F. Rossi,
W. D. Richardson, J.-F. Brunet.
The sacral autonomic outflow is sympathetic Science

Marina Soulika, Anna-Lila Kaushik, Benjamin Mathieu, Raquel Lourenço1, Anna Z. Komisarczuk, Sebastian Alejo Romano, Adrien Jouary, Alicia Lardennois, Nicolas Tissot, Shinji Okada, Keiko Abe, Thomas S. Becker and Marika Kapsimali.
Diversity in cell motility reveals the dynamic nature of the formation
of zebrafish taste sensory organs Development

Philippe Foerster, Marie Daclin, Shihavuddin Asm, Marion Faucourt, Alessandra Boletta, Auguste Genovesio, Nathalie Spassky.
mTORC1 signaling and primary cilia are required for brain ventricle morphogenesis Development

Plamont MA, Billon-Denis E, Maurin S, Gauron C, Pimenta FM, Specht CG, Shi J, Quérard J, Pan B, Rossignol J, Morellet N, Volovitch M, Lescop E, Chen Y, Triller A, Vriz S, Le Saux T, Jullien L, Gautier A.
Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo. Proc Natl Acad Sci U S A. 2016 Jan 19 ;113(3):497-502. doi : 10.1073/pnas.1513094113. Epub 2015 Dec 28.

Wang L, Dumoulin A, Renner M, Triller A, Specht CG (2016) The Role of Synaptopodin in Membrane Protein Diffusion in the Dendritic Spine Neck. PLoS ONE 11(2)

Guy Bouvier, David Higgins, Maria Spolidoro, Damien Carrel, Benjamin Mathieu, Clément Léna, Stéphane Dieudonné, Boris Barbour, Nicolas Brunel and Mariano Casado, The role of presynaptic NMDA receptors in the plasticity rule at the parallel fibre-Purkinje cell synapse Cell report, accepted for publication

2015

Bourbousse, C., Mestiri, I., Zabulon, G., Bourge, M., Formiggini, F., Koini, M. A., Brown, S. C., Fransz, P., Bowler, C. and Barneche, F. Light signaling controls nuclear architecture reorganization during seedling establishment. Proc. Natl. Acad. Sci. USA. 112 : E2836-44 (2015).

Jourdon, A., Gresset, A., Spassky, N., Charnay, P., Topilko, P., Santos, R. Prss56, a novel marker of adult neurogenesis in the mouse brain. Brain Struct Funct. (2015) Epub ahead of print.

W. Akemann, J.-F. Léger, C. Ventalon, B. Mathieu, S. Dieudonné and L. Bourdieu, Fast spatial beam shaping by acousto-optic diffraction for 3D non-linear microscopy, Opt. Express 23(22), 28191-28205 (2015)

Gresset A, Coulpier F, Gerschenfeld G, Jourdon A, Matesic G, Richard L, Vallat J-M, Charnay P and Topilko P. Boundary Caps Give Rise to Neurogenic Stem Cells and Terminal Glia in the Skin. Stem Cell Reports 5, 278–290 (2015)

Tanaka, A., de Martino, A., Amato, A., Montsant, A., Mathieu, B., Rostaing, P., Tirichine, L. and Bowler, C. Ultrastructure and membrane traffic during cell division in the marine pennate diatom Phaeodactylum tricornutum. Protist 166 : 506-521 (2015).

Morrissey, J., Sutak, R., Paz-Yepes, J., Atsuko, T., Moustafa, A., Veluchamy, A., Thomas, Y., Botebol, H., Bouget, F.-Y., McQuaid, J. B., Tirichine, L., Allen, A. E., -* Lesuisse, E. and Bowler, C. A novel protein, ubiquitous in marine phytoplankton, concentrates iron at the cell surface and facilitates ferric ion uptake. Curr. Biol. 25 : 364-371 (2015).

Labalette C, Wassef M A, Desmarquet-Trin Dinh C, Bouchoucha Y-X, Le Men J, Charnay P and Gilardi-Hebenstreit P. Molecular dissection of segment formation in the developing hindbrain. Development 142, 185-195 (2015)

Bailleul, B., Berne, N., Murik, O., Petroutsos, D., Prihoda, J, Tanaka, A., Villanova, V., Bligny, R., Flori, S., Falconet, D., Krieger-Liszkay, A., Santabarbara, S., Rappaport, F., Joliot, P., Tirichine, L., Falkowski, P. G., Cardol, P., Bowler, C. and Finazzi, G. Energetic coupling between plastids and mitochondria drives CO2 assimilation in diatoms. Nature 524 : 366-369 (2015).

Huysman, M. J. J., Tanaka, A., Bowler, C., Vyverman, W. and De Veylder, L. Functional characterization of the diatom cyclin-dependent kinase A2 as a mitotic regulator reveals plant-like properties in a non-green lineage. BMC Plant Biology 15:86 (2015).

Shrivastava AN, Redeker V, Fritz N, Pieri L, Almeida LG, Spolidoro M, Liebmann T, Bousset L, Renner M, Léna C, Aperia A, Melki R, Triller A. α-synuclein assemblies sequester neuronal α3-Na+/K+-ATPase and impair Na+ gradient. EMBO J. 2015 Oct 1 ;34(19):2408-23.

Salvatico C, Specht CG, Triller A. Synaptic receptor dynamics : from theoretical concepts to deep quantification and chemistry in cellulo. Neuropharmacology. 2015 Jan ;88:2-9.

2014

Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire E, Morin X, Livet J. Multiplex cell and lineage tracking with combinatorial labels. Neuron. 2014 Feb 5 ;81(3):505-20.

Pont-Lezica L, Beumer W, Colasse S, Drexhage H, Versnel M, Bessis A. Microglia shape corpus callosum axon tract fasciculation : functional impact of prenatal inflammation. Eur J Neurosci. 2014 May ;39(10):1551-7.

Béchade C, Colasse S, Diana MA, Rouault M, Bessis A. Glia. NOS2 expression is restricted to neurons in the healthy brain but is triggered in microglia upon inflammation. 2014 Jun ;62(6):956-63

Al Jord A, Lemaître AI, Delgehyr N, Faucourt M, Spassky N, Meunier A Centriole amplification by mother and daughter centrioles differs in multiciliated cells.. Nature. 2014 Dec 4 ;516(7529):104-7

Petkovic M et al. The SNARE Sec22b has a non-fusogenic function in plasma membrane expansion. Nat Cell Biol 2014 ; 16:434-44.

Saadaoui M, Machicoane M, di Pietro F, Etoc F, Echard A, Morin X. Dlg1 controls planar spindle orientation in the neuroepithelium through direct interaction with LGN. J Cell Biol. 2014 Sep 15 ;206(6):707-17.

Espinosa-Medina I, Outin E, Picard C.A, Chettouh Z, Dymecki S, Consalez G.G, Coppola E, Brunet JF. Parasympathetic ganglia derive from Schwann cell precursors. Science (2014) 10.1126

Squarzoni P, Oller G, Hoeffel G, Pont-Lezica L, Rostaing P, Low D, Bessis A, Ginhoux F, Garel S. Microglia modulate wiring of the embryonic forebrain. Cell Rep. 2014 Sep 11 ;8(5):1271-9. 2.

Machicoane M, de Frutos CA, Fink J, Rocancourt M, Lombardi Y, Garel S, Piel M, Echard A. SLK-dependent activation of ERMs controls LGN-NuMA localization and spindle orientation. J Cell Biol. 2014 Jun 23 ;205(6):791-9.

Gouzer G, Specht CG, Allain L, Shinoe T, Triller A. Benzodiazepine-dependent stabilization of GABA(A) receptors at synapses. Mol Cell Neurosci. 2014 Nov ;63:101-13.

2013

Lokmane L, Proville R, Narboux-Nême N, Györy I, Keita M, Mailhes C, Léna C, Gaspar P, Grosschedl R, Garel S. Sensory map transfer to the neocortex relies on pretarget ordering of thalamic axons. Curr Biol. 2013 May 6 ;23(9):810-6.

Shrivastava AN, Kowalewski JM, Renner M, Bousset L, Koulakoff A, Melki
R, Giaume C, Triller A. β-amyloid and ATP-induced diffusional trapping
of astrocyte and neuronal metabotropic glutamate type-5 receptors. Glia.
2013 Oct ;61(10):1673-86

Hennekinne L, Colasse S, Triller A, Renner M. Differential control of
thrombospondin over synaptic glycine and AMPA receptors in spinal cord
neurons. J Neurosci. 2013 Jul 10 ; 33(28):11432-9

Dang I, Gorelik R, Sousa-Blin C, Derivery E, Guérin C, Linkner J,
Nemethova M, Dumortier JG, Giger FA, Chipysheva TA, Ermilova VD, Vacher
S, Campanacci V,Herrada I, Planson AG, Fetics S, Henriot V, David V,
Oguievetskaia K, Lakisic G, Pierre F, Steffen A, Boyreau A, Peyriéras N,
Rottner K, Zinn-Justin S, Cherfils J,Bièche I, Alexandrova AY, David NB,
Small JV, Faix J, Blanchoin L, Gautreau A. Inhibitory signalling to the
Arp2/3 complex steers cell migration. Nature. 2013 Nov 14 ;503(7475):281-4.

Lokmane L, Proville R, Narboux-Nême N, Györy I, Keita M, Mailhes C, Léna
C, Gaspar P, Grosschedl R, Garel S. Sensory map transfer to the
neocortex relies on pretarget ordering of thalamic axons. Curr Biol.
2013 May 6 ;23(9):810-6.

Deck M, Lokmane L, Chauvet S, Mailhes C, Keita M, Niquille M, Yoshida M,
Yoshida Y, Lebrand C, Mann F, Grove EA, Garel S. Pathfinding of
corticothalamic axons relies on a rendezvous with thalamic projections.
Neuron. 2013 Feb 6 ;77(3):472-84.

2012

Dumortier JG, Martin S, Meyer D, Rosa FM, David NB. Collective
mesendoderm migration relies on an intrinsic directionality signal
transmitted through cell contacts. Proc Natl Acad Sci USA. 2012 Oct 16 ;
109 (42) : 16945-50.

2011

Kapsimali M, Kaushik AL, Gibon G, Dirian L, Ernest S, Rosa FM. Fgf
signaling controls pharyngeal taste bud formation through miR-200 and
Delta-Notch activity. Development. 2011 Aug ;138(16):3473-84.

Bielle F, Marcos-Mondéjar P, Leyva-Díaz E, Lokmane L, Mire E, Mailhes C,
Keita M, García N, Tessier-Lavigne M, Garel S, López-Bendito G. Emergent
growth cone responses to combinations of Slit1 and Netrin 1 in
thalamocortical axon topography. Curr Biol. 2011 Oct 25 ;21(20):1748-55.

Liens utiles

Fatima MELOUKI (microscopie photonique) — 2015-04-21T12:22:31Z

Formation

Vidéo-conférence
iBiology
La mission de iBiology est de transmettre, sous la forme de vidéos gratuites en libre accès, la passion de la biologie. Vous trouverez des présentations utiles pour la compréhension aux techniques de microscopie confocale.

Animation vidéo et documentation écrite
Olympus Microcopy Resource Center
Il s'agit d'un fabricant d'équipement optique. Vous trouverez sur leur site des présentation concernant les techniques de microscopie.

Outils

Spectre de fluorescence
Fluoscout, Spectraviewer, Fluorescence Spectrum Viewer.
Sur ces sites, vous pouvez retrouver le spectre d'excitation et d'émission de vos fluorophores.

Logiciel à télécharger

Acquisition d'images
LAS x
Il s'agit d'un logiciel d'acquisition d'images développé par Leica. Si vous souhaitez avoir le CD d'installation veuillez nous le demander directement.

Analyse d'images
Icy
Il s'agit d'un logiciel d'analyse d'image développé par une équipe de l'Institut Pasteur.

FIJI
Il s'agit d'un logiciel de traitement d'images fonctionnant avec ImageJ, Bio-Format, Oméro, µmanager.

ImageJ
Il s'agit d'un logiciel de traitement d'images de référence.

Imaris MAC / Imaris converter MAC
Imaris Windows / Imaris converter Windows
Il s'agit d'un logiciel de traitement et de visuation d'images de référence. Le format des images doit être convertie en .ims