IBENS - ENS

Gaspard Gerschenfeld

Gaspard Gerschenfeld (équipe Charnay) — 2017-11-27T10:44:44Z

Training

2013-2015 Neurology resident
2009-2010 Master Degree in stem cell biology (UPMC)
2007-2013 Medical student (UPMC)

Research topic

Use of skin precursor cells to regenerate the central nervous system

Fanny COULPIER

Gaspard Gerschenfeld (équipe Charnay) — 2015-02-18T09:29:07Z

Fanny COULPIER
IE
01 44 32 39 76
E-Mail

Training

2002 : BTS in biotechnology

2004 : Assistant Engineer at the Ecole Normale Supérieur, INSERM U784, in Patrick Charnay's lab

2004-2007 : « Diploma of EPHE » , at the Ecole Normale Supérieur, INSERM U784 , in Patrick Charnay's lab.

2007-2010 : Ph.D in Development Biology at the Ecole Normale Supérieur, Paris, in Patrick Charnay's lab. Thesis : Functionnal analysis of boundary cap cells ans Schwann cells during the peripheral nervous system development.

2010- : Engineer, at the Ecole Normale Supérieur, Paris, U1024 in Patrick Charnay's lab

Research topics

Molecular and cellular characterisation of boundary cap-derived stem cells in peripheral nervous sytem.

Recent publications

Cthrc1 is a negative regulator of myelination in Schwann cells.
Apra C, Richard L, Coulpier F, Blugeon C, Gilardi-Hebenstreit P, Vallat JM, Lindner V, Charnay P, Decker L.Glia. 2012 Mar ;60(3):393-403.

Boundary cap cells are peripheral nervous system stem cells that can be redirected into central nervous system lineages.
Zujovic V, Thibaud J, Bachelin C, Vidal M, Deboux C, Coulpier F, Stadler N, Charnay P, Topilko P, Baron-Van Evercooren A.
Proc Natl Acad Sci U S A. 2011 Jun 28 ;108(26):10714-9. doi : 10.1073/pnas.1018687108. Epub 2011 Jun 13.

Krox20 inactivation in the PNS leads to CNS/PNS boundary transgression by central glia.
Charnay P, Coulpier F, Decker L, Funalot B, Vallat JM, Garcia-Bragado F, Topilko P.
Bull Acad Natl Med. 2010 Apr-May ;194(4-5):743-4. French.

Secreted amyloid precursor protein β and secreted amyloid precursor protein α induce axon outgrowth in vitro through Egr1 signaling pathway.
Chasseigneaux S, Dinc L, Rose C, Chabret C, Coulpier F, Topilko P, Mauger G, Allinquant B.PLoS One. 2011 Jan 27 ;6(1):e16301. doi : 10.1371/journal.pone.0016301.

Krox20 inactivation in the PNS leads to CNS/PNS boundary transgression by central glia.
Coulpier F, Decker L, Funalot B, Vallat JM, Garcia-Bragado F, Charnay P, Topilko P.
Rev Neurol (Paris). 2011 Jan ;167(1):51-6. doi : 10.1016/j.neurol.2010.07.043. Epub 2010 Dec 28.

CNS/PNS boundary transgression by central glia in the absence of Schwann cells or Krox20/Egr2 function.
Coulpier F, Decker L, Funalot B, Vallat JM, Garcia-Bragado F, Charnay P, Topilko P.
J Neurosci. 2010 Apr 28 ;30(17):5958-67. doi : 10.1523/JNEUROSCI.0017-10.2010.

Boundary cap cells are highly competitive for CNS remyelination : fast migration and efficient differentiation in PNS and CNS myelin-forming cells.
Zujovic V, Thibaud J, Bachelin C, Vidal M, Coulpier F, Charnay P, Topilko P, Baron-Van Evercooren A.
Stem Cells. 2010 Mar 31 ;28(3):470-9. doi : 10.1002/stem.290.

Novel features of boundary cap cells revealed by the analysis of newly identified molecular markers.
Coulpier F, Le Crom S, Maro GS, Manent J, Giovannini M, Maciorowski Z, Fischer A, Gessler M, Charnay P, Topilko P.
Glia. 2009 Oct ;57(13):1450-7. doi : 10.1002/glia.20862.

Patrick Torbey

Gaspard Gerschenfeld (équipe Charnay) — 2014-10-28T15:41:02Z

PhD student
01 44 32 39 79
E-Mail

Training

2012-2014 : Master Degree in Genetics at Paris VII Diderot

2011-2012 : Bachelor in Genetics at Paris VII Diderot

2007-2011 : Bachelor in Medical Sciences at Paris V Descartes

Research topics

Regulation of a developmental gene of the vertebrate hindbrain

Katarzyna Radomska

Gaspard Gerschenfeld (équipe Charnay) — 2014-10-28T15:38:20Z

Postdoc
01 44 32 37 14
E-Mail

Training

2014 : Postdoctoral researcher in Patrick Charnay's lab at ENS, Paris

2009 - 2014 : Ph.D. in Developmental Biology at Uppsala University, Dept. of Evolution and Development, Sweden. Thesis : “Functional studies of the Quaking gene. Focus on astroglia and neurodevelopment”

2007 - 2009 : Research training in the laboratories of Dr. Åsa Fex Svenningsen and Prof. Helgi Schiöth at Dept. of Neuroscience, Uppsala University, Sweden

2003 - 2008 : M. Sc. in Biology with specialisation in Neurobiology at Jagiellonian University, Dept. of Neuroanatomy, Krakow, Poland

Research topic

Molecular and cellular characterisation of boundary cap-derived stem cells in the mouse dermis.

Publications

Radomska KJ, Halvardson J, Reinius B, Lindholm Carlström E, Emilsson L, Feuk L, Jazin E. RNA-binding protein QKI regulates Glial Fibrillary Acidic Protein expression in human astrocytes. Hum Mol Genet. 2013 Apr 1 ;22(7):1373-82.

Reinius B, Johansson MM, Radomska KJ, Morrow EH, Pandey GK, Kanduri C, Sandberg R, Williams RW, Jazin E. Abundance of female-biased and paucity of male-biased somatically expressed genes on the mouse X-chromosome. BMC Genomics. 2012 Nov 10 ;3:607.

Olszewski PK, Fredriksson R, Eriksson JD, Mitra A, Radomska KJ, Gosnell BA, Solvang MN, Levine AS, Schiöth HB. Fto colocalizes with a satiety mediator oxytocin in the brain and upregulates oxytocin gene expression. Biochem Biophys Res Commun. 2011 May 13 ;408(3):422-6.

Olszewski PK, Radomska KJ, Ghimire K, Klockars A, Ingman C, Olszewska AM, Fredriksson R, Levine AS, Schiöth HB. Fto immunoreactivity is widespread in the rodent brain and abundant in feeding-related sites, but the number of Fto-positive cells is not affected by changes in energy balance. Physiol Behav. 2011 May 3 ;103(2):248-53.

Reinius B, Shi C, Hengshuo L, Sandhu KS, Radomska KJ, Rosen GD, Lu L, Kullander K, Williams RW, Jazin E. Female-biased expression of long non-coding RNAs in domains that escape X-inactivation in mouse. BMC Genomics. 2010 Nov 3 ;11:614.

Jiang L, Saetre P, Radomska KJ, Jazin E, Lindholm Carlström E. QKI-7 regulates expression of interferon-related genes in human astrocyte glioma cells. PLoS One. 2010 Sep 29 ;5(9). pii : e13079.

Olszewski PK, Fredriksson R, Olszewska AM, Stephansson O, Alsiö J, Radomska KJ, Levine AS, Schiöth HB. Hypothalamic FTO is associated with the regulation of energy intake not feeding reward. BMC Neurosci. 2009 Oct 27 ;10:129.

Élodie Thierion

Gaspard Gerschenfeld (équipe Charnay) — 2014-10-28T15:36:11Z

PhD Student
01 44 32 30 61
E-Mail

Training

2007-2009 1st and 2nd year preparation class for BCPST at Lycée Chaptal (Paris)

2009-2010 Degree in Molecular and Cellular Biology from the University Paris XI

2010-2012 Master degree in Genetics, Cellular Biology and Development at the University of Oslo and the University of Paris XI

Research topics

Genetic and epigenetic mechanisms involved in the regulation of a developmental gene of the posterior brain.

Alexandre Jourdon

Gaspard Gerschenfeld (équipe Charnay) — 2014-10-28T15:33:01Z

PhD student
+33 1 44 32 39 82
E-Mail

Training

2005-2010 : Engineering degree from the National Institute of Applied Sciences in Lyon (INSA de Lyon).
2010:Internship in the Exploratory Unit of Sanofi-Aventis, Montpellier.
2010-2011 : Neurosciences Master 2, Paris 6

Research topics

Analysis of neural stem cell biology in mouse.

Aurélie Gresset

Gaspard Gerschenfeld (équipe Charnay) — 2014-10-28T15:29:40Z

Postdoc
33 1 44 32 39 79
E-Mail

Training

2010 - : Postdoc in Patrick Charnay's lab.
2005 – 2010 : Ph.D in Pharmacology, University of North Carolina at Chapel Hill, USA in Dr. John Sondek laboratory elucidating the molecular mechanisms of phospholipase C-gamma activation
2002 – 2005 : Bachelor of science in biochemistry, Clemson University, South-Carolina, USA

Research topics

Role of Krox20 in cellular segregation between the central nervous system and the peripheric nervous system.

Publications

Aurélie Gresset, Stephanie N. Hicks, T. Kendall Harden, and John Sondek. Mechanism of phosphorylation-induced activation of phospholipase C-γ isozymes. J Biol Chem, 2010, Nov 12 ;285(46):35836-47.
Sabrice Guerrier, Jaeda Coutinho-Budd, Takayuki Sassa, Aurélie Gresset, Nicole Vincent Jordan, Adam Frost, and Franck Polleux. srGAP2 regulates neuronal migration and morphogenesis through the ability of its F-BAR domain to induce membrane protrusions. Cell, 2009, Sep 4 ;138(5):990-1004.

Carole DESMARQUET

Gaspard Gerschenfeld (équipe Charnay) — 2014-10-28T14:40:43Z

Engineer
33 1 44 32 39 82
E-Mail

Training / Positions

1986 : CES in industrial microbiology
1995 : DEST in biology , CNAM
1986-1988 : Assistant Engineer at the Rhône Poulenc Research Center, « Tératology in vitro » laboratory
1988-1989 : Assistant Engineer at the Alfred Fournier Institut, « Experimental Syphilis » laboratory
1989-1994 : Assistant Engineer at the Institut Pasteur, INSERM U190, « Human viral oncology » laboratory
1994- : Engineer at the Ecole Normale Supérieure, INSERM U784 ,« Molecular Genetic of Development »

Research topics

Regulation of the activity of the transcription factor Krox20 during development of the nervous system in vertebrates.

Publications

Breitburd F., Kirnbauer R., Hubbert N.L., Nonnenmacher B., Trin-Dinh-Desmarquet C., Orth G.,Schiller J.T.and Lowy D.R. Immunization with viruslike particles from cottontail rabbit papillomavirus (CRPV) can protect against expérimental CRPV (1995) infection. J. Virol., 69, 3959-3963.
Topilko P., Levi G., Merlo G., Mantero S., Desmarquet C., Mancardi G. and Charnay P. Differential regulation of the zinc finger genes Krox-20 and Krox-24 (Egr-1) suggests antagonistic roles in Schwann cells. (1997)J. Neurosci Res., 50, 702-712.
Seitanidou T., Schneider-Maunoury S., Desmarquet C., Wilkinson D G., and Charnay P. Krox-20 is a key regulator of rhombomere-specific gene expression in the developing hindbrain.(1997) Mech Dev., 65, 31-42.
Helmbacher F., Pujades C., Desmarquet C., Frain M., Rijli F.M., Chambon P and Charnay P. Hoxa 1 and Krox-20 synergize to control the development of rhombomere 3 (1998). Develop., 125, 4539-4748.
Ghislain, J., Desmarquet-Trin-dinh, C., Jaegle, M., Meijer, D., Charnay, P. and Frain, M. Characterisation of cis-acting sequences reveals a biphasic, axon-dependent regulation of Krox20 during Schwann cell development. (2002). Development, 129, 155-166.
Ghislain, J., Desmarquet-Trin-Dinh, C., Gilardi-Hebenstreit, P., Charnay, P. and Frain, M. Neural crest patterning : autoregulatory and crest-specific elements co-operate for Krox20 transcriptional control (2003). Development, 130, 941-953.
Decker, L., Desmarquet-Trin-Dinh, C., Taillebourg, E., Ghislain, J., Vallat J.-M. and Charnay, P. Peripheral myelin maintenance is a dynamic process requiring constant Krox20 expression. (2006) J. Neuroscience, Sep 20 ;26(38):9771-9.
Pouilhe M., Gilardi-Hebenstreit P., Desmarquet-Trin Dinh C. and Charnay P. Direct regulation of vHnf1 by retinoic acid signalling and several MAF-related factors in the neural tube. (2007) Developmental biology, 309, 344-357.
Wassef, M., Chomette, D., Pouilhe, M., Stedman, A., Havis, E., Desmarquet-Trin Dinh, C., Schneider-Maunoury, S., Gilardi-Hebenstreit, P., Charnay, P. and Ghislain, J. Rostral hindbrain patterning involves direct activation of a Krox20 transcriptional enhancer by Hox/Pbx and Meis factors. (2008). Development, 135(20):3369-78.

Rouben Nagalingum

Gaspard Gerschenfeld (équipe Charnay) — 2014-10-28T14:38:27Z

Fonction : Accounting development section

IBENS - Section Développement
Ecole Normale Supérieure
46 rue d'Ulm
75230 Paris cedex 05 (France)
Tel : 33 (0)1 44 32 39 77
Fax : 33 (0)1 44 32 39 88
E-Mail

Training

1998 Maitrise AES (social and economic administration), Paris VII.

1999 Master, Ecole Supérieure de Gestion

Renata SANTOS

Gaspard Gerschenfeld (équipe Charnay) — 2014-10-28T14:34:46Z

Staff scientist (CR1 CNRS)
(33)-1-44-32-35-73
E-Mail

Training

1991. Degree in Biology from University of Lisbon, Portugal

1995. Ph.D. in Molecular Biology with first class honors from University of Lisbon, Portugal

1995-2000. Post-doctoral fellow at the Institut Jacques Monod (Danièle Touati's lab, Paris) : study of the role of the bacterial superoxide dismutase during the symbiosis of Sinorhizobium meliloti and the leguminous plant alfalfa.

200-2002. Post-doctoral fellow at the Institut Jacques Monod (Jean-Michel Camadro's lab, Paris) : study of iron homeostasis and oxidative stress in the yeast S. cerevisiae.

2002. Recruited as Investigator (Chargé de Recherche) at the CNRS in France.

2002-2011. Work in the same lab (IJM) on the effect of frataxin-deficiency (that cause a neurodegenerative disease in humans) on oxidative stress response and cell death in yeast and mammalian cells.

2012-. Move to Patrick Charnay's lab at the ISBN to study adult neuronal stem cells in mice.

Research topics

– Adult neurogenesis
– Cellular oxidative stress response

Recent Publications

– S. Lefevre, C. Brossas, F. Auchère, N. Boggetto, J.M. Camadro and R. Santos (2012) Apn1 AP-endonuclease is essential for the repair of oxidatively damaged DNA bases in yeast frataxin-deficient cells. Hum. Mol. Genet. 21, 4060-4072.

– S. Lefevre, D. Sliwa, P. Rustin, J.M. Camadro and R. Santos (2012) Oxidative stress induces mitochondrial fragmentation in frataxin-deficient cells. Biochem. Biophys. Res. Comms. 418, 336-341.

– D. Sliwa, J. Dairou, J.M. Camadro and R. Santos (2012) Inactivation of mitochondrial aspartate aminotransferase contributes to the respiratory deficit of yeast frataxin-deficient cells. Biochem. J. 441, 945-953.

– S. Lefevre, D. Sliwa, F. Auchère, C. Brossas, C. Ruckenstuhl, N. Boggetto, E. Lesuisse, F. Madeo, J.M. Camadro and R. Santos (2012) The yeast metacaspase is implicated in oxidative stress response in frataxin-deficient cells. FEBS Lett. 586, 143-148.

– Bayot, R. Santos, J.M. Camadro and P. Rustin (2011) Friedreich ataxia : the vicious circle hypothesis revisited. BMC Med. 9, 112.

– Seguin, R. Santos, D. Pain, A. Dancis, J.M. Camadro and E. Lesuisse (2011) Co-precipitation of phosphate and iron limits mitochondrial phosphate availability in Saccharomyces cerevisiae lacking the yeast frataxin homologue (YFH1). J. Biol. Chem. 286, 6071-6079.

– R. Santos, S. Lefevre, D. Sliwa, A. Seguin, J.M. Camadro and E. Lesuisse (2010) Friedreich's ataxia : molecular mechanisms, redox considerations and therapeutic opportunities. Antioxid. Redox Signal. 13, 651-690.

– Seguin, R. Sutak, A.L. Bulteau, R. Garcia-Serres, J.L. Oddou, S. Lefevre, R. Santos, A. Dancis, J.M. Camadro, J.M. Latour and E. Lesuisse (2010) Evidences that yeast frataxin is not an iron storage protein in vivo. Biochim. Biophys. Acta - Mol. Basis Dis. 1802, 531-538.