Publications
Oligoadenylated mRNAs are detected and degraded by the nonsense-mediated mRNA decay
Gouhier T, Cornille A, Gauthier E, Saveanu C, Badis G.
In preparation
Deadenylation rate is not a major determinant of RNA degradation in yeast
Audebert L, Feuerbach F, Decourty L, Namane A, Permal E, Badis G, Saveanu C. (2024).
EMBO
Identification of an evolutionary conserved binding motif responsible for the recruitment of NMD factors to the UPF1 helicase
Barbarin-Bocahu I, Ulryck N, Rigobert A, Ruiz-Gutierrez N, Decourty L, Raji M, Garkhal B, Le Hir H, Saveanu C, Graille M. (2024).
PLOS Biology
Exon-junction complex association with stalled ribosomes and slow translation-independent disassembly
Bensaude O, Barbosa I, Morillo L, Dikstein R, Le Hir H. (2024).
Nature Communications
Comprehensive mapping of exon junction complex binding sites reveals universal EJC deposition in Drosophila
Morillo L, Paternina T, Alasseur Q, Genovesio A, Schwartz S, Le Hir H. (2023).
BMC Biology
Exclusion of m6A from splice-site proximal regions by the exon junction complex dictates m6A topologies and mRNA stability
Uzonyi A, Dierks D, Nir R, Kwon OS, Toth U, Barbosa I, Burel C, Brandis A, Rossmanith W, Le Hir H, Slobodin B, Schwartz S. (2023).
Molecular Cell
Novel approaches to study helicases using magnetic tweezers
Ruiz-Gutierrez N, Rieu M, Ouellet J, Allemand J-F, Croquette V, Le Hir H (2022)
Methods in Enzymology
Exon junction complex dependent mRNA localization is linked to centrosome organization during ciliogenesis
Kwon OS, Mishra R, Safieddine A, Coleno E, Alasseur Q, Faucourt M, Barbosa I, Bertrand E, Spassky N, Le Hir H. (2021).
Nature Communications
NCBP3 positively impacts mRNA biogenesis
Dou Y, Barbosa I, Jiang H, Iasillo C, Molloy KR, Schulze WM, Cusack S, Schmid M, Le Hir H, LaCava J, Jensen TH (2020)
Nucleic Acids Research
A localization screen reveals translation factories and widespread co-translational protein targeting
Chouaib R, Saffieddine A, Pichon X, Kwon OS, Samacoits A, Traboulsi AM, Tsanov N, Robert MC, Poser I, Zimmer C, Hyman AA, Le Hir H, Walter T, Zibara K, Mueller F, Peter M, and Bertrand E (2020)
Develomental Cell
Structural and functional insights into CWC27/CWC22 heterodimer linking the exon junction complex to spliceosomes
Busetto V, Barbosa I, Basquin J, Marquenet É, Hocq R, Hennion M, Paternina JA, Namane A, Conti E, Bensaude O, Le Hir H (2020)
Nucleic Acids Research
ALFA : annotation landscape for aligned reads
Bahin M, Noël BF, Murigneux V, Bernard C, Bastianelli L, Le Hir H, Lebreton A, Genovesio A (2019)
BMC genomics
Antisense transcriptional interference mediates condition-specific gene repression in budding yeast
Nevers A, Doyen A, Malabat C, Néron B, Kergrohen T, Jacquier A, Badis G. (2018)
Nucleic Acids Research
Nonsense-mediated mRNA decay involves two distinct Upf1-bound complexes
Dehecq M, Decourty L, Namane A, Proux C, Kanaan J, Le Hir H, Jacquier A, Saveanu C (2018)
EMBO Journal
Monitored eCLIP : high accuracy mapping of RNA-protein interactions
Hocq R, Paternina J, Alasseur Q, Genovesio A, Le Hir H (2018)
Nucleic Acids Research
UPF1-like helicase grip on nucleic acids dictates processivity
Kanaan J, Raj S, Decourty L, Saveanu C, Croquette V, Le Hir H (2018)
Nature Communications
Exon Junction Complexes can have distinct functional flavours to regulate specific splicing events
Wang Z, Ballut L, Barbosa I, Le Hir H (2018)
Scientific Reports
HTLV-1 Tax plugs and freezes UPF1 helicase leading to Nonsense-Mediated mRNA Decay inhibition
Fiorini F, Robin JP, Kanaan J, Borowiak M, Croquette V, Le Hir H, Jalinot P, Mocquet V. (2018)
Nature Communications.
A conserved structural element in the RNA helicase UPF1 regulates its catalytic activity in an isoform-specific manner
Gowravaram M, Bonneau F, Kanaan J, Maciej VD, Fiorini F, Raj S, Croquette V, Le Hir H, Chakrabarti S. (2018)
Nucleic Acids Research.
A mechanistic study of helicases with magnetic traps.
Hodeib S, Raj S, Manosas M, Zhang W, Bagchi D, Ducos B, Fiorini F, Kanaan J, Le Hir H, Allemand JF, Bensimon D, Croquette V. (2017)
Protein Science
Mutations in the Spliceosome Component CWC27 Cause Retinal Degeneration with or without Additional Developmental Anomalies.
Xu M, Xie YA, Abouzeid H, et al. (2017)
American Journal of Human Genetics
Insights into the design and interpretation of iCLIP experiments.
Haberman N, Huppertz I, Attig J, König J, Wang Z, Hauer C, Hentze MW, Kulozik AE, Le Hir H, Curk T, Sibley CR, Zarnack K, Ule J. (2017)
Genome Biology
The exon junction complex as a node of postranscriptional networks.
Le Hir H, Saulière J, Wang Z. (2016)
Nature Review in Molecular and Cellular Biology.
Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities.
Fiorini F, Bagchi D, Le Hir H*, Croquette V*. (2015)
Nature Communications.
*Corresponding authors
CLIP-seq to discover transcriptome-wide imprinting of RNA binding proteins in living cells.
Saulière J, Le Hir H. (2015)
Methods Molecular Biology.
Transcriptome-wide modulation of splicing by the Exon Junction Complex.
Wang Z, Murigneux V, Le Hir H. (2014)
Genome Biol.
Crystal structure of the human eIF4AIII-CWC22 complex shows how a DEAD-box protein is inhibited by a MIF4G domain.
Buchwald G, Schüssler S, Basquin C, Le Hir H, Conti E. (2013)
Proc Natl Acad Sci U S A
Transcriptome-wide identification of RNA binding sites by CLIP-seq.
Murigneux V, Saulière J, Roest Crollius H, Le Hir H. (2013)
Methods
EJC core component MLN51 interacts with eIF3 and activates translation.
Chazal PE, Daguenet E, Wendling C, Ulryck N, Tomasetto C, Sargueil B, Le Hir H. (2013)
Proc Natl Acad Sci U S A
Tight intramolecular regulation of the human Upf1 helicase by its N- and C-terminal domains.
Fiorini F, Boudvillain M, Le Hir H. (2013)
Nucleic Acids Res.
CLIP-seq of eIF4AIII reveals transcriptome-wide mapping of the human exon junction complex.
Saulière J, Murigneux V, Wang Z, Marquenet E, Barbosa I, Le Tonquèze O, Audic Y, Paillard L, Roest Crollius H, Le Hir H. (2012)
Nature structural & molecular biology
Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.
Barbosa I, Haque N, Fiorini F, Barrandon C, Tomasetto C, Blanchette M, Le Hir H. (2012)
Nature structural & molecular biology
The human T-lymphotropic virus type 1 tax protein inhibits nonsense-mediated mRNA decay by interacting with INT6/EIF3E and UPF1.
Mocquet V, Neusiedler J, Rende F, Cluet D, Robin JP, Terme JM, Duc Dodon M, Wittmann J, Morris C, Le Hir H, Ciminale V, Jalinot P. (2012)
J Virol.
Perispeckles are major assembly sites for the exon junction core complex.
Daguenet E, Baguet A, Degot S, Schmidt U, Alpy F, Wendling C, Spiegelhalter C, Kessler P, Rio MC, Le Hir H, Bertrand E, Tomasetto C. (2012)
Mol Biol Cell.
Proteins associated with the exon junction complex also control the alternative splicing of apoptotic regulators.
Michelle L, Cloutier A, Toutant J, Shkreta L, Thibault P, Durand M, Garneau D, Gendron D, Lapointe E, Couture S, Le Hir H, Klinck R, Elela SA, Prinos P, Chabot B. (2012)
Mol Cell Biol.
Biochemical characterization of the RNA helicase UPF1 involved in nonsense-mediated mRNA decay.
Fiorini F, Bonneau F, Le Hir H. (2012)
Methods in Enzymology
Molecular mechanisms for the RNA-dependent ATPase activity of Upf1 and its regulation by Upf2.
Chakrabarti S, Jayachandran U, Bonneau F, Fiorini F, Basquin C, Domcke S, Le Hir H, Conti E (2012)
Mol Cell
The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex.
Murachelli AG, Ebert J, Basquin C, Le Hir H, Conti E (2011)
Nature structural & molecular biology
The exon junction complex differentially marks spliced junctions.
Saulière J, Haque N, Harms S, Barbosa I, Blanchette M, Le Hir H, (2010)
Nature structural & molecular biology
Insights into the recruitment of the NMD machinery from the crystal structure of a core EJC-UPF3b complex.
Buchwald G, Ebert J, Basquin C, Sauliere J, Jayachandran U, Bono F, Le Hir H, Conti E, (2010)
Proceedings of the National Academy of Sciences of the United States of America
A UPF3-mediated regulatory switch that maintains RNA surveillance.
Chan WK, Bhalla AD, Le Hir H, Nguyen LS, Huang L, Gécz J, Wilkinson MF, (2009)
Nature structural & molecular biology
Mechanism of ATP turnover inhibition in the EJC.
Nielsen KH, Chamieh H, Andersen CB, Fredslund F, Hamborg K, Le Hir H, Andersen GR, (2009)
RNA (New York, N.Y.)
EJCs at the heart of translational control.
Le Hir H, Séraphin B, (2008)
Cell
Structural insights into the exon junction complex.
Le Hir H, Andersen GR, (2008)
Current opinion in structural biology
NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity.
Chamieh H, Ballut L, Bonneau F, Le Hir H, (2008)
Nature structural & molecular biology
The exon-junction-complex-component metastatic lymph node 51 functions in stress-granule assembly.
Baguet A, Degot S, Cougot N, Bertrand E, Chenard MP, Wendling C, Kessler P, Le Hir H, Rio MC, Tomasetto C, (2007)
Journal of cell science
Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies.
Buchet-Poyau K, Courchet J, Le Hir H, Séraphin B, Scoazec JY, Duret L, Domon-Dell C, Freund JN, Billaud M, (2007)
Nucleic acids research
Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.
Andersen CB, Ballut L, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Séraphin B, Le Hir H, Andersen GR, (2006)
Science (New York, N.Y.)
The exon junction core complex is locked onto RNA by inhibition of eIF4AIII ATPase activity.
Ballut L, Marchadier B, Baguet A, Tomasetto C, Séraphin B, Le Hir H, (2005)
Nature structural & molecular biology
Expression of GFRalpha1 receptor splicing variants with different biochemical properties is modulated during kidney development.
Charlet-Berguerand N, Le Hir H, Incoronato M, di Porzio U, Yu Y, Jing S, de Franciscis V, Thermes C, (2004)
Cellular signalling
Association of the breast cancer protein MLN51 with the exon junction complex via its speckle localizer and RNA binding module.
Degot S, Le Hir H, Alpy F, Kedinger V, Stoll I, Wendling C, Seraphin B, Rio MC, Tomasetto C, (2004)
The Journal of biological chemistry
Splicing enhances translation in mammalian cells : an additional function of the exon junction complex.
Nott A, Le Hir H, Moore MJ, (2004)
Genes & development
DcpS can act in the 5 -3 mRNA decay pathway in addition to the 3 -5 pathway.
van Dijk E, Le Hir H, Séraphin B, (2003)
Proceedings of the National Academy of Sciences of the United States of America
How introns influence and enhance eukaryotic gene expression.
Le Hir H, Nott A, Moore MJ, (2003)
Trends in biochemical sciences
5 exon interactions within the human spliceosome establish a framework for exon junction complex structure and assembly.
Reichert VL, Le Hir H, Jurica MS, Moore MJ, (2002)
Genes & development
5 -End RET splicing : absence of variants in normal tissues and intron retention in pheochromocytomas.
Le Hir H, Charlet-Berguerand N, de Franciscis V, Thermes C, (2002)
Oncology
The protein Mago provides a link between splicing and mRNA localization.
Le Hir H, Gatfield D, Braun IC, Forler D, Izaurralde E, (2001)
EMBO reports
The DExH/D box protein HEL/UAP56 is essential for mRNA nuclear export in Drosophila.
Gatfield D, Le Hir H, Schmitt C, Braun IC, Köcher T, Wilm M, Izaurralde E, (2001)
Current biology : CB
The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay.
Le Hir H, Gatfield D, Izaurralde E, Moore MJ, (2001)
The EMBO journal
The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions.
Le Hir H, Izaurralde E, Maquat LE, Moore MJ, (2001)
The EMBO journal
Relative expression of the RET9 and RET51 isoforms in human pheochromocytomas.
Le Hir H, Charlet-Berguerand N, Gimenez-Roqueplo A, Mannelli M, Plouin P, de Franciscis V, Thermes C, (2000)
Oncology
Pre-mRNA splicing alters mRNP composition : evidence for stable association of proteins at exon-exon junctions.
Le Hir H, Moore MJ, Maquat LE, (2000)
Genes & development
